Spatial and Temporal Amperometric Monitoring Dopamine Release from Single PC12 Cells with Carbon Fiber Nanoelectrode

Abstract

Abstract: Carbon fiber nanoelectrodes(200-300 nm) were firstly used to amperometricaly monitor the dopamine release from single PC12 cells with temporal resolution and especially more higher spatial resolution than those obtained by using microelectrodes. When the nanoelectrode was in a distance 1 μm above the PC12 cell, only one peak signal corresponding to a single vesicle exocytotic event was detected caused from the stimulation with 1 mmol/L nicotine. The spatial difference of exocytosis was also detected by placing the electrode onto the different locations of the cell body, the results have demostrated that the spatial distribution of dopamine in cells is not uniform and the time for stimulating secretion is very different. Nanoelectrodes electrochemical method can provide a powerful tool for observing the temporal and spatial characteristics of the secretion from single cells directly.

Key words: Carbon fiber nanoelectrodes, Single cells, Single vesicles, Dopamine, Quantum release

CLC Number:

O657.1

TrendMD:

HUANG Wei-Hua, ZHANG Li-Yao, CHENG Wei, PANG Dai-Wen WANG Zong-Li, CHENG Jie-Ke . Spatial and Temporal Amperometric Monitoring Dopamine Release from Single PC12 Cells with Carbon Fiber Nanoelectrode[J]. Chem. J. Chinese Universities, 2003, 24(3): 425.

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Spatial and Temporal Amperometric Monitoring Dopamine Release from Single PC12 Cells with Carbon Fiber Nanoelectrode

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