Chem. J. Chinese Universities ›› 2015, Vol. 36 ›› Issue (7): 1269.doi: 10.7503/cjcu20150273
• Analytical Chemistry • Previous Articles Next Articles
FENG Juanjuan, ZHAO Yiman, WANG Haiyan*()
Received:
2015-04-07
Online:
2015-07-10
Published:
2015-06-11
Contact:
WANG Haiyan
E-mail:hywang@mail.ahnu.edu.cn
Supported by:
CLC Number:
TrendMD:
FENG Juanjuan, ZHAO Yiman, WANG Haiyan. Colorimetric Detection of Dopamine Based on Silver Nanoparticles†[J]. Chem. J. Chinese Universities, 2015, 36(7): 1269.
Fig.4 Visual color changes of AgNPs solution in the presence of different concentrations of DA(A), calibration curve for detection of DA(B) and effect of DA concentration on the peak position(C)(A) cDA/(μmol·L-1); a. 0; b. 0.05; c. 1; d. 4; e. 7; f. 10; g. 13; h. 16; i. 19; j. 22; k. 25; l .28.Inset of (B): plots of the absorbance of AgNPs vs. DA concentrations ranging from 0.05 μmol/L to 31 μmol/L.
NPs Probe | Linear range/(μmol·L-1) | LOD/(μmol·L-1) | Ref. |
---|---|---|---|
AuNPs | 0.5—10 | 0.20 | [ |
AHMT-AuNPs* | 0.2—1.1 | 0.07 | [ |
Au nanorods | 0.2—12 | 0.047 | [ |
AgNPs | 0—0.6 | 0.06 | [ |
AgNPs-Ag+ | 0.05—16 | 0.04 | This work |
Table 1 Comparison of analytical features of colorimetric methods for detection of DA
NPs Probe | Linear range/(μmol·L-1) | LOD/(μmol·L-1) | Ref. |
---|---|---|---|
AuNPs | 0.5—10 | 0.20 | [ |
AHMT-AuNPs* | 0.2—1.1 | 0.07 | [ |
Au nanorods | 0.2—12 | 0.047 | [ |
AgNPs | 0—0.6 | 0.06 | [ |
AgNPs-Ag+ | 0.05—16 | 0.04 | This work |
Fig.5 Changes in the absorbance of AgNPs with 10 μmol/L DA or other analytes(A) and UV-Vis spectra(B) of AgNPs with 10 μmol/L DA(a) and 100 μmol/L AA(b)(A) a. DA; b. Lys; c. Phe; d. Gly; e. Glu; f. UA; g. CA; h. Catechol; i. K+; j. Na+; k. Cu2+. Inset of (B): visual color changes.
Sample | Added/(μmol·L-1) | Measured/(μmol·L-1) | Recovery(%) | RSD(%) |
---|---|---|---|---|
Serum 1 | 0.3 | 0.296 | 98.7 | 3.0 |
Serum 2 | 0.3 | 0.31 | 103.3 | 6.4 |
Serum 3 | 0.3 | 0.294 | 98 | 3.0 |
Table 2 Detection of dopamine in human serum(n=5)
Sample | Added/(μmol·L-1) | Measured/(μmol·L-1) | Recovery(%) | RSD(%) |
---|---|---|---|---|
Serum 1 | 0.3 | 0.296 | 98.7 | 3.0 |
Serum 2 | 0.3 | 0.31 | 103.3 | 6.4 |
Serum 3 | 0.3 | 0.294 | 98 | 3.0 |
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