Studies on a Microfluidic Chip Based on the Continuous Flow PCR Amplification System

Abstract

Abstract: A PCR amplification system comprising a syringe pump sample processor, a microfluidic PCR chip and a 3-temperature-zone heating block was developed in this work. The system is capable of performing continuous PCR amplification with a low carryover between neighboring samples. The substrate thickness and thermal insulation of the temperature control unit were optimized. A 24-cycle PCR amplification of a 500-base pair fragment from λ-DNA was successfully performed with a total reaction time of 4 9 min.

Key words: PCR amplification, Microfluidic chip, DNA

CLC Number:

O657

TrendMD:

LIU Jin-Hua, YIN Xue-Feng, XU Guang-Ming, FANG Zhao-Lun, CHEN Huai-Zeng . Studies on a Microfluidic Chip Based on the Continuous Flow PCR Amplification System[J]. Chem. J. Chinese Universities, 2003, 24(2): 232.

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Studies on a Microfluidic Chip Based on the Continuous Flow PCR Amplification System

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