Abstract: Anew denoising method for HPLC, named filtering algorithm based on computing analysis of spectra(FACAS), was proposed.At the measuring wavelength and with regard to the higher signal-to-noise ratio(S/N), the data points the values of which are above the median of the chromatographic peak height, were selected out.Then every spectrum corresponding to these points from HPLC/DAD was processed to acquire a standard unit spectrum.According to the relationship between the spectra and the standard unit spectrum, the denoising chromatographic data were obtained by reconstructed computation using the least squares algorithm.The results of simulated and practical experiment both indicate that the proposed method can effectively filter noise and it has a better performance of denoising compared with median filter.The facts show that FACASIs a powerful denoising tool for HPLC/DAD data.

Key words: HPLC, Denoise, Spectrum, Filter