Rapid Determination of Albumin of Urine in ΔI Measurement Mode with Portable Spectrophotometer of High Sensitivity

Abstract

Abstract: Aportable spectrophotometer developed by Changchun R & D Center for Analytical Instruments, which combines ΔImeasurement mode and a measurement mode together with a high sensitivity, good accuracy and low cost, is employed to replace the traditional spectrophotometer and the sensitivity is enhanced. Therefore, a new rapid method with low cost and simplicity in operation for protein determination is developed. For this method, the linear range is shown to be 0—0.74 μg/mL, the detection limit is 0.01 μg/mL, and the analysis can be completed within 5 min. The albumin in urine is determined and all the results are confirmed by the standard method. Because of the rapid analysis speed, less expenditure, more convenience and simplicity in operation of this method, it is possible to use this method to diagnose glycosuric nephropathy at an early stage, even do it at home.

Key words: Portable spectrophotometer, ΔI measurement mode, Albumin, Urine, Rapid determination

CLC Number:

O657.32

TrendMD:

CHEN Huan-Wen, JIN Qin-Han, Yang-Li-Quan, Luo-Gui-Min . Rapid Determination of Albumin of Urine in ΔI Measurement Mode with Portable Spectrophotometer of High Sensitivity[J]. Chem. J. Chinese Universities, 2001, 22(6): 928.

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Rapid Determination of Albumin of Urine in ΔI Measurement Mode with Portable Spectrophotometer of High Sensitivity

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