The Microstructure Analysis of Enzymes Immobilized by Sol-gel Method and Its Application in Phenol Optical Chemical Sensor

Abstract

Abstract: The external states of horseradish peroxidase(HRP) encapsulated membrane prepared by enclosed aging method and dip coating method are compared by common optical microscopy to clarify that the sol gel membrane prepared by enclosed aging method is no cracking. The transmission electron microscopy(TEM) is used to observe the character of the sol gel derived HRPmembrane. The TEMphotos show that enzyme can uniformly distribute in the gel as if in the solution and do not leach from the gel. This membrane can be used to fabricate a phenol optical sensor based on the decrease of chemiluminescence intensity. The response mechanism is discussed by compete reaction.

Key words: Sol gel, Horseradish peroxidase, Microstructure, Phenol, Optical sensor

CLC Number:

O657

TrendMD:

LI Jun, WANG Ke-Min, XIAO Dan, YANG Xiao-Hai . The Microstructure Analysis of Enzymes Immobilized by Sol-gel Method and Its Application in Phenol Optical Chemical Sensor[J]. Chem. J. Chinese Universities, 2000, 21(7): 1018.

References

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