Ultrasensitive Fluorometric Detection of Oligosaccharides as Their APTS Derivatives by Capillary Electrophoresis

Abstract

Abstract: Reducing oligosaccharides, after reductive amination, were separated and detected as their APTSderivatives by capillary electrophoresis with laser-induced fluorescence. Brief optimization studies have also been performed with respect to CEseparation conditions of oligosaccharides. The oligosaccharide ladders were analyzed in an acidic phosphate-tris buffer(pH2.5) or in the alkaline borate buffer. Acid hydrolysis products of dextran T-40 labeled with APTSwere also successfully mapped.

Key words: 8-Aminopyrene-1,3,6-trisufonate(APTS), Oligosaccharide, Dextran, Capillary electropho-resis with laser-induced fluorescence detection

CLC Number:

O657.8

TrendMD:

DANG Fu-Quan, CHEN Yi, GUO Qing, XU Gui-Yun . Ultrasensitive Fluorometric Detection of Oligosaccharides as Their APTS Derivatives by Capillary Electrophoresis[J]. Chem. J. Chinese Universities, 2000, 21(2): 206.

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Ultrasensitive Fluorometric Detection of Oligosaccharides as Their APTS Derivatives by Capillary Electrophoresis

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