Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica†

doi:10.7503/cjcu20170232

Abstract

Abstract:

The sulfated fuco-oligosaccharides were prepared via acid hydrolysis degradation of crude fucoidan extracted from Saccharina japonica. Through the optimization of degradation and precipitation conditions, the hydrolysis and sedimentation law of fucoidan were studied. Physicochemical analysis showed that the supernatant was heterozygous sulfated fuco-oligosaccharide(fucose>55%). Four fractions of fucose based low polyme-rization degree of oligosaccharides were purified by Bio-Gel P4 low-pressure permeation chromatography, and the fucose content of the fractions was up to 90% according to monosaccharide composition analysis and electrospray ionization mass spectrometry(ESI-MS) analysis. Thus, the fractions were series of low-polymerized sulfated fuco-oligosaccharide/fucose, and the major fractions could be expressed with the formula of Fuc_1~3S_1~3, Fuc_1~2S_1~2, Fuc_1~2S, and FucGlcAS.

Key words: Fucoidan, Fuco-oligosaccharide, Acid hydrolysis

CLC Number:

O629

TrendMD:

GENG Lihua, JIN Weihua, WANG Jing, ZHANG Quanbin. Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica^†[J]. Chem. J. Chinese Universities, 2017, 38(12): 2193.

Figures/Tables 4

Table 1 Condition setting of degradation of crude fucoidan from seaweed

Condition	Level
Condition	1	2	3
Hydrolysis time/h	3	5	7
Precipitation reagent	EtOH	AcN	(CH₃)₂CO
Concentration(%)	50	70	90

Fig.1 Monosaccharides composition of SPF1(mass ratio) a. 1-1-1; b. 1-2-1; c. 1-3-1; d. 2-1-1; e. 2-2-1; f. 2-3-1; g. 3-1-1; h. 3-2-1; i. 3-3-1; j. 1-1-2; k. 1-2-2; l. 1-3-2; m. 2-1-2; n. 2-2-2-; o. 2-3-2; p. 3-1-2; q. 3-2-2; r. 3-3-2; s. 1-1-3; t. 1-2-3; u. 1-3-3; v. 2-1-3; w. 2-2-3; x. 2-3-3; y. 3-1-3; z. 3-2-3; z. 3-3-3.

Fig.2 Separation graph(A) and TLC analysis for fractions FP1—FP4(B) of SPF1 by LPGPC (B) Std.: standard of fucose; lanes 1—4: FP1—FP4.

Table 2 Negative ion ESI-MS data of fucoidan-derived sulfated oligosaccharide fragments FP1—FP4

Fraction	Degree of polymerization	Structure	Found ion(H form)
Fraction	Degree of polymerization	Structure	[M-H]^-	[M-2H]^2-	[M-3H]^3-
FP1	1	FucS	243.0
	1	FucS₂	323.0	161.0
	2	FucGlcAS	419.2
	2	Fuc₂S	389.0
	2	Fuc₂S₂	469.0	234.0
	2	Fuc₂S₃	549.0	274.0	182.3
	3	Fuc₃S	535.1
	3	Fuc₃S₂	615.0	307.0
FP2	1	FucS	243.0
	1	FucS₂	323.0	161.0
	2	Fuc₂S	389.0
	2	Fuc₂S₂	469.0	234.0
FP3	1	FucS	243.0
	2	Fuc₂S	389.0
FP4	1	FucS	243.0

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