The Solution Structure of Cytochrome b5 Mutant(E44A/E48A/E56A/D60A)

Chem. J. Chinese Universities ›› 2000, Vol. 21 ›› Issue (11): 1631.

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The Solution Structure of Cytochrome b₅ Mutant(E44A/E48A/E56A/D60A)

WU Yi-Bing^1,3, LU Jun^1,3, QIAN Cheng-Ming¹, LI Er-Cheng², WANG Jin-Feng², TANG Wen-Xia¹, ZHU De-Xi³, WANG Yun-Hua⁴, WANG Wen-Hu⁴, LU Jun-Xia⁴, XIE Yi⁴, HUANG Zhong-Xian⁴

1. State Key Laboratoryof Coordination Chemistry, Nanjing University, Nanjing 210093, China;
2. National Laboratoryof Biomacromolecules, Academic Sinica, Beijing 100101, China;
3. State Key Laboratoryof Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China;
4. Departmentof Chemistry, Fudan University, Shanghai 200433, China

Received:2000-05-16 Online:2000-11-24 Published:2000-11-24

Abstract

Abstract: The solution structure of bovine cytochrome b₅ mutant (E44A/E48A/E56A/D60A) has been obtained from ¹H NMR spectra measured at 600 MHz. The final RMSD values with respect to the average structure are (0.056±0.009) nm and (0.105±0.010) nm for backbone and all heavy atoms, respectively. The solution structure, when compared with the X-ray structure of wild type, has no significant changes in the whole folding and secondary structure. Small changes are restricted to local conformation near mutated sites.

Key words: Cytochrome b₅, Mutant, NMR, Solution structure

CLC Number:

Q518.2

TrendMD:

WU Yi-Bing, LU Jun, QIAN Cheng-Ming, LI Er-Cheng, WANG Jin-Feng, TANG Wen-Xia, ZHU De-Xi, WANG Yun-Hua, WANG Wen-Hu, LU Jun-Xia, XIE Yi, HUANG Zhong-Xian. The Solution Structure of Cytochrome b₅ Mutant(E44A/E48A/E56A/D60A)[J]. Chem. J. Chinese Universities, 2000, 21(11): 1631.

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The Solution Structure of Cytochrome b₅ Mutant(E44A/E48A/E56A/D60A)

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