Isolation, Characterization and Properties of Spirulina Glycoprotein

Abstract

Abstract: Aglycoprotein was isolated from Chinese spirulina. Purification of this glycoprotein was accomplished by foam in a loop bubble column and gel chromatography on Sephacryl S-100.The homogeneity of this glycoprotein was proven by gel filtration on Sepha-dex G-200 and SDS-PAGE. The molecular weight of the glycoprotein was 130000. Characterization of this glycoprotein by IR analysis showed the typical absorption of polysaccharide and indicated the presence of α-glycoside linkage. β-Elimination reaction demonstrated the existence of ο-glycosidic linkage in this spirulina glycoprotein.

Key words: Spirulina, Glycoprotein, Polysaccharide

CLC Number:

Q539

TrendMD:

YIN Gang, LIU Zheng, LI Chen, LIU Fei, DING Fu-Xin, YUAN Nai-Ju . Isolation, Characterization and Properties of Spirulina Glycoprotein[J]. Chem. J. Chinese Universities, 1999, 20(4): 565.

[1]	BAO Han, LUO Jing, SHI Lianxin, XU Fujian, WANG Shutao. Fabricating Polysaccharide Micro-hydrogel via Superhydrophobic Pillar-structured Si Substrate ^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1484.
[2]	WANG Tingting, LI Yuan, YANG Lili, BAO Changhao, CHENG Han. In vivo Dynamic Detection of Aloe Polysaccharides Using Carbon Fiber Microelectrodes Modified with Gold Nanoparticles ^† [J]. Chem. J. Chinese Universities, 2020, 41(1): 87.
[3]	JIN Xin,JI Guoli,ZHAO Xiaoliang,LI Guoyun,YU Guangli. Sulfated Arabinose from Codium fragile: Preparation and Extensive Structural Characterization^† [J]. Chem. J. Chinese Universities, 2019, 40(4): 733.
[4]	LI Cheng,WANG Chengjian,JIN Wanjun,HAN Jianli,YANG Meifang,GAO Xi,HUANG Linjuan,WANG Zhongfu. Mass Spectrometric Analysis of N-Glycans of Glycoprotein Separated by SDS-PAGE Gel from Ginkgo Seed^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 69.
[5]	LING Yao, LIU Xuejing, HAO Haijing, HAO Xiaohui, BAI Libin, WU Yonggang. Synthesis and Properties of Water-soluble Glycosyl Fluorescent Polymer with Aggregation-induced Emission Effect^† [J]. Chem. J. Chinese Universities, 2018, 39(6): 1319.
[6]	GAO Yunxiao,HU Xiaolong,WANG Yuerong,JIANG Zhihong,ZHANG Hongyang,ZHANG Min,HU Ping. Primary Structural Analysis of Dendrobium officinale Polysaccharide^† [J]. Chem. J. Chinese Universities, 2018, 39(5): 934.
[7]	LIU Benguo, LIU Jiangwei, LI Jiaqi, GENG Sheng, MO Haizhen, LIANG Guizhao. 3D-QSAR and Interaction Mechanism of Flavonoids s P-glycoprotein Inhibitors^† [J]. Chem. J. Chinese Universities, 2017, 38(1): 41.
[8]	PENG Yifang, WANG Chengjian, WANG Jingjing, LI Lingmei, JIN Wanjun, QIANG Shan, SHI Hongdan, ZHANG Ying, HUANG Linjuan, WANG Zhongfu. Analysis of Antigenic Determinant Glycans of Peanut Allergy Glycoprotein Ara h1 by Mass Spectrometry^† [J]. Chem. J. Chinese Universities, 2016, 37(9): 1622.
[9]	ZHAO Qi, HE Wanying, DUAN Lijie, ZHANG Yu, YU Shuangjiang, GAO Guanghui. Fabrication and Characterization of Injectable Polysaccharide-polypeptide Hydrogel Based on Schiff’s Base^† [J]. Chem. J. Chinese Universities, 2016, 37(9): 1750.
[10]	SHEN Yanhong, CHEN Linxiao, ZHANG Wenqing, XU Zhizhen, WAN Yanfei, ZHANG Li, XIA Wei. Structural Characterization of Mannatide^† [J]. Chem. J. Chinese Universities, 2016, 37(6): 1088.
[11]	ZHI Zijian, ZOU Mingming, LI Shan, CHEN Jianle, YE Xingqian, CHEN Shiguo. Rheological and Structural Characterization of Pectin Polysaccharides from Citrus Pulp^† [J]. Chem. J. Chinese Universities, 2016, 37(6): 1175.
[12]	LIU Yang, YIN Lu, GONG Guiping, PENG Yifang, HUANG Linjuan, WANG Zhongfu. Structural Characterization, Antioxidant Activity and Immunomodulatory Activity of the Polysaccharide LRLP3 from Leaves of Lycium ruthenicum Murra^† [J]. Chem. J. Chinese Universities, 2016, 37(2): 261.
[13]	FENG Jilu, QI Junru, LIU Qianru. Fabrication of Soy Protein Isolate-soluble Soy Polysaccharide Core-shell Nanogels via Maillard Reaction and Self-assembly^† [J]. Chem. J. Chinese Universities, 2016, 37(11): 1999.
[14]	GUAN Qingxiang, ZHANG Guangyuan, SUN Dandan, SUN Shilin, SUN Cheng, LIU Xin, HAN Bing. Investigation on Synthesis of Bletilla Striata Polysaccharides Amphiphilic Polymer and Drug-loaded Delivery System^† [J]. Chem. J. Chinese Universities, 2016, 37(10): 1915.
[15]	YIN Junyi, LIU Xiaoying, NIE Shaoping, XIE Mingyong. Solution, Conformational Properties and Morphology of Psyllium Polysaccharide with Ferulic Acid Removal^† [J]. Chem. J. Chinese Universities, 2016, 37(1): 43.

Isolation, Characterization and Properties of Spirulina Glycoprotein

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments