Analysis of Antigenic Determinant Glycans of Peanut Allergy Glycoprotein Ara h1 by Mass Spectrometry†

doi:10.7503/cjcu20160147

Abstract

Abstract:

Peanut seed total protein and glycoprotein Ara h1, which can cause allergic reactions, were chosen as the target materials in this study. Firstly, the glycans were released and labeled with 1-phenyl-3-methyl-5-pyrazolone(PMP) by recently developed “one-pot” method. Subsequently, the glycans were fractionated and enriched through C18 solid phase extraction column. Finally, the obtained glycans were analysed by electrospray ionization mass spectrometry(ESI-MS), MSⁿ and Hydrophilic interaction liquid chsomatography-mass spectrometry(HILIC-MS). The results indicated that Ara h1 has ten N-glycans, including seven high-mannose type, two ones with xylose modification, and one allergenic core α1,3-fucosylated N-glycan, the content of which is 12.45% of total glycans.

Key words: Peanut allergy, Allergenic glycoprotein Ara h1, Glycan structure, Electrospray ionization mass spectrometry(ESI-MS), Hydrophilic interaction liquid chsomatography-mass spectrometry(HILIC-MS)

CLC Number:

TrendMD:

PENG Yifang, WANG Chengjian, WANG Jingjing, LI Lingmei, JIN Wanjun, QIANG Shan, SHI Hongdan, ZHANG Ying, HUANG Linjuan, WANG Zhongfu. Analysis of Antigenic Determinant Glycans of Peanut Allergy Glycoprotein Ara h1 by Mass Spectrometry^†[J]. Chem. J. Chinese Universities, 2016, 37(9): 1622.

Figures/Tables 4

References 22

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Monosaccharide composition	m/z	t/min	Release method	Type
H3N2	1241.58^a 1263.33^b 1279.08^c	29.55	PNGase F PNGase A One-pot	Core
H3N2X1	1373.42^a 1395.42^b	36.68	PNGase F PNGase A One-pot	Complex
H4N2	1425.42^b	39.77	PNGase F PNGase A One-pot	High-mannose
H3N2F1X1	1541.33^b	54.76	PNGase A One-pot	Complex
H4N2X1	1557.42^b	46.55	PNGase F PNGase A One-pot	Hybrid
H5N2	1587.42^b	52.95	PNGase F PNGase A One-pot	High-mannose
H6N2	1749.50^b	64.14	PNGase F PNGase A One-pot	High-mannose
H7N2	1911.42^b	73.91	PNGase F PNGase A One-pot	High-mannose
H8N2	1045.37^d	84.21	PNGase F PNGase A One-pot	High-mannose
H9N2	1126.08^d	88.96	PNGase F PNGase A One-pot	High-mannose

Monosaccharide composition	m/z	t/min	Release method	Type
H3N2	1241.58^a 1263.33^b 1279.08^c	29.55	PNGase F PNGase A One-pot	Core
H3N2X1	1373.42^a 1395.42^b	36.68	PNGase F PNGase A One-pot	Complex
H4N2	1425.42^b	39.77	PNGase F PNGase A One-pot	High-mannose
H3N2F1X1	1541.33^b	54.76	PNGase A One-pot	Complex
H4N2X1	1557.42^b	46.55	PNGase F PNGase A One-pot	Hybrid
H5N2	1587.42^b	52.95	PNGase F PNGase A One-pot	High-mannose
H6N2	1749.50^b	64.14	PNGase F PNGase A One-pot	High-mannose
H7N2	1911.42^b	73.91	PNGase F PNGase A One-pot	High-mannose
H8N2	1045.37^d	84.21	PNGase F PNGase A One-pot	High-mannose
H9N2	1126.08^d	88.96	PNGase F PNGase A One-pot	High-mannose