Matrixassisted Laser Desorption/Ionization Mass Spectral Study of Saccharides

Abstract

Abstract: Matrixassisted laser desorption/ionization (MALDI) mass spectra of saccharides with three common matrixes were studied here. It is shown that Na⁺and K⁺play important roles in the determination of oligasaccharide molecule weight and the molecular weights(MW) of glucans(dextran) with MW more than 10 000 is determined successfully with the help of column chromatography. By comparing the effects of three matrixes in saccharide analysis and comparing positive ion and negative ion matrixassisted laser desorption/ionization mass spectra of oligo- and polysaccharides, the most suitable matrix for saccharide analysis, 2,5-dihydroxylbezonic acid (DHB), is selected and the ion formation processes of saccharides under matrix-assisted laser adsorption/ionization condition are clarified.

Key words: Matrixassisted laser desorption/ionization mass spectrometry, Oligosaccharide, Polysaccharide, Molecular weight

CLC Number:

O629.1

TrendMD:

HAO Chun-Yan, MA Xiu-Li, LIU Zhi-Qiang, JI Yi-Ping, LIU Shu-Ying, LIU Chang-Cheng, SUN Yun-Xiu, LIU Ju-Zheng. Matrixassisted Laser Desorption/Ionization Mass Spectral Study of Saccharides[J]. Chem. J. Chinese Universities, 1998, 19(7): 1090.

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Matrixassisted Laser Desorption/Ionization Mass Spectral Study of Saccharides

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