Abstract: In this paper,the reversed phase suspension and embedment techniques were adopted of preparing the paramagnetic cellulose beads (MCBs). While the viscose suspended in oil phase was heated at 90 ℃ for 40 min, cellulose might be regenerated from cellulose xanthate,and the γ-Fe₂O₃ superfine powder(φ<300 nm) dispersed in the viscose was wrapped up among the regenerated cellulose chains, then the MCBs were obtained whose mean size might be less than 300μm, and its distribution was narrow.Moist MCBs have the porosity more than 85%. Their magnetizability was of the order of magnitude of 10^-3 emu/g. These MCBs can be activated by many kinds of common methods, and can be applied to immobilizing different bioactive ligands to obtain various magnetic affinity adsorbents(MAA). We activated the MCBs in a 35 mmol/L sodium periodate solution, and immobilized the human chorionic gonadotropin(hCG) on them to obtain the MAAwith 300 IUhCG/g content. This kind of MAA could specifically adsorb anti-hCG antibody from murine ascites supernatant.

Key words: Cellulose, Magnetic resin, Affinity adsorbent, Human chorionic gonadotropin (hCG)