Studies on the interaction of Photosensitizers and Glucose Oxidase Using Capillary Electrophoresis

Abstract

Abstract: The interaction of glucose oxidase (GOD) and four photosensitizers, ZnpcS₄,ZnPc(NHCH₂COOH)₄, CIAIPcS, and porphyrine-like, were monitored by capillaryelectrophoresis. GOD, its degenerated products and photosensitizers were simultaneouslyseparated in a buffer of 300 mmol/Lborate at PH8.5 on a commercial CEinstrument. Bycomparison of the electropherograms of GODas well as the mixture of GODand photosensitizerswithout/with irradiation of different time, it was found that photosensitized oxidationsbetween GODand different photosensitizers have different reaction mechanisms and kinetics.

Key words: Capillary electrophoresis, Glucose oxidase, Photosensitized oxidation

CLC Number:

O657

TrendMD:

HU Ping, LUO Guo-An, WANG Ru-Ji, DONG Run-An, SONG Xin-Qi. Studies on the interaction of Photosensitizers and Glucose Oxidase Using Capillary Electrophoresis[J]. Chem. J. Chinese Universities, 1997, 18(9): 1459.

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Studies on the interaction of Photosensitizers and Glucose Oxidase Using Capillary Electrophoresis

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