A Preliminary Study on the Possibility of Screening Small Peptide in Peptide Library

Abstract

Abstract: Peptide libraries have been successfully used in the field concerning molecular interactions,such as antigen-antibody,receptor-ligand,enzyme-inhibitor and so on.These research results show that a few critical residues which can form hydrogen bond or ionic bond in proteins make a major contribution to the interactions of proteins.This paper reports the possibility of screening short peptide in phage hexa-peptide library using DGGSAA as a model.The results show that it is possible to screen the short peptide containing the residues forming hydrogen bond and ionic bond in peptide library,which improved the specificity of screening greatly and the recovery of phage reduced from 0.001 %to 0.00022%.That indicates that the interactions between short peptides is strong enough to survive the screening and it supposes a simple model to research molecular recognition with peptide libraries.

Key words: Peptide library, Screen, Phage, Peptide

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QI Jie, FANG Rui, ZHOU Hui, LI Wei, SHEN Jia-Cong. A Preliminary Study on the Possibility of Screening Small Peptide in Peptide Library[J]. Chem. J. Chinese Universities, 1996, 17(11): 1738.

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A Preliminary Study on the Possibility of Screening Small Peptide in Peptide Library

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