Effects of Curcumin on Growth and Oxide Regulation of Esophageal Cancer KYSE410 Cells†

doi:10.7503/cjcu20190129

Abstract

Abstract:

This work took esophageal cancer KYSE410 cells as the research object. [3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) method and some other techniques were used to study the effect of curcumin on growth and metabolism of KYSE410 cells. The results show that curcumin strongly inhibited growth of KYSE410 cells and half maximal inhibitory concentration(IC₅₀) was 17.9 μmol/L. Besides, the level of superoxide dismutase(SOD) decreases to 43.2% and the malondialdehyde(MDA) increases to 125.1%. Also, the level of lactate decreases to 44.4% and the activity of lactate dehydrogenase decreases to 58.2% and the activity of pyruvate kinase increases to 216.7%. It is presumed that curcumin has strong suppressed effect on this kind of cell. Curcumin can interfere oxidative stress and inhibit glycolysis by decreasing activity of lactate dehydrogenase and increasing activity of pyruvate kinase.

Key words: Curcumin, Esophageal cancer, KYSE410 cell

CLC Number:

O621
Q932

TrendMD:

ZHOU Xinyuan,HONG Jiaxin,HE Shaobing,ZHANG Lianru. Effects of Curcumin on Growth and Oxide Regulation of Esophageal Cancer KYSE410 Cells^†[J]. Chem. J. Chinese Universities, 2019, 40(7): 1405.

Figures/Tables 4

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c(Curcumin)/(μmol·L^-1)	Time/h	Inhibition ratio(%)
6.25	60	4.12±0.42
12.5	60	33.60±0.48
25	60	80.05±0.91
50	60	96.73±2.38
100	60	98.45±2.01

c(Curcumin)/(μmol·L^-1)	Time/h	Inhibition ratio(%)
6.25	60	4.12±0.42
12.5	60	33.60±0.48
25	60	80.05±0.91
50	60	96.73±2.38
100	60	98.45±2.01

Sample	c(SOD)/ (ng·mL^-1)	c(MDA)/ (ng·mL^-1)	c(LDH)/ (U·mg^-1)	c(PK)/ (U·mg^-1)	c(Lactate)/ (ng·mL^-1)
DMSO	44.46±6.30	12.01±1.62	5.25±0.01	60.32±8.87	(0.140±3.8)×10^-5
10 μmol/L Curcumin	36.12±4.67	16.01±2.52	4.00±0.05	93.48±2.15	(0.127±6.7)×10^-9
20 μmol/L Curcumin	28.39±4.09	21.02±2.53	2.63±0.03	130.19±3.65	(0.126±8.1)×10^-5
40 μmol/L Curcumin	24.22±3.39	27.63±3.01	1.99±0.03	190.44±3.72	(0.041±4.8)×10^-6

Sample	c(SOD)/ (ng·mL^-1)	c(MDA)/ (ng·mL^-1)	c(LDH)/ (U·mg^-1)	c(PK)/ (U·mg^-1)	c(Lactate)/ (ng·mL^-1)
DMSO	44.46±6.30	12.01±1.62	5.25±0.01	60.32±8.87	(0.140±3.8)×10^-5
10 μmol/L Curcumin	36.12±4.67	16.01±2.52	4.00±0.05	93.48±2.15	(0.127±6.7)×10^-9
20 μmol/L Curcumin	28.39±4.09	21.02±2.53	2.63±0.03	130.19±3.65	(0.126±8.1)×10^-5
40 μmol/L Curcumin	24.22±3.39	27.63±3.01	1.99±0.03	190.44±3.72	(0.041±4.8)×10^-6