Laser Opto-thermal On-line Detection for the Gel Capillary Electrophoresis

Abstract

Abstract: The opto-thermal detection technique based on crossed-beam thermal lens effect has been developed for the on-line determination of capillary polyacrylamide gel electrophoresis(PAGE).The minimum detectable concentration of methylene blue in ethanol filled into 220 pm i.d.capillary was obtained to be 1.76×10^-9 mol/L,corresponding to the absorbance of 3.8×10^-6.Amixture of bromophenol blue and amide black was separated by capillary PAGE and detected by laser opto-thermal on-line detection technique.Mass detection limit for bromophenol blue injected into the gel capillary was found to be 40 pg.

Key words: Laser, Opto-thertnal detection, Capillary electrophoresis

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DENG Yan-Zhuo, YU Pei, YE Meng-Wei, LIU Heng-Biao. Laser Opto-thermal On-line Detection for the Gel Capillary Electrophoresis[J]. Chem. J. Chinese Universities, 1992, 13(11): 1386.

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Laser Opto-thermal On-line Detection for the Gel Capillary Electrophoresis

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