A Jack Bean Tissue Electrode for Urea Determination

Abstract

Abstract: A jack bean tissue section electrode sensitive to urea has been prepared by coupling a jack bean slice with an ammonia gas-sensing probe using PVC membrane pHelectrode as the internal element. The potentiometric response characteristics of this new electrode under steady-state and dynamic condition were examined. The steady-state linear response ranse of this electrode is 1.0×10^-2～4.6 ×10^-5mol/L, with a detection limit of 4.0×10^-5mol/L. The influence of some experimental parameters including buffer composition, operation pH, etc. are discussed. Urea was determined by using dynamic method, with an average recovery of 98.9% and a standard deviation of 1.8%.

Key words: Biosensor, Tissue section electrode, Determination of urea, Jack bean section electrode

TrendMD:

Shen Guoli, Ma Ming, Zeng Geming, Yu Ruqin, Xie Jinyun, Zhang Meifen. A Jack Bean Tissue Electrode for Urea Determination[J]. Chem. J. Chinese Universities, 1989, 10(3): 308.

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A Jack Bean Tissue Electrode for Urea Determination

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