Construction and Assembly Kinetics of Amino Acid-Iron Nano-assemblies

doi:10.7503/cjcu20240385

Abstract

Abstract:

We employed supramolecular interactions to fabricate amino acid-iron nano-assemblies using β-iron hydroxide oxide as the building block. The dynamics of assembly at various temperatures was investigated， shedding light on how temperature influences the nucleation， growth， and assembly processes. By optimizing the assembly conditions， a shuttle-shaped nano-assembly with a length of 179.1 nm and a width of 36.1 nm was successfully prepared， featuring a total iron（Fe） content of 49.6%， with complex Fe accounting for 32.1%. Cell experiments demonstrated that this unique amino acid-iron nano-assembly could be efficiently uptaken by human bladder transitional cell carcinoma T24 cells and inhibited their proliferation， with a half maximal inhibitory concentration （IC₅₀） of 163.8 μg/mL. These findings suggest that the nano-assembly possesses excellent tumor therapeutic potential and holds significant promise for research in the field of nanomedicine.

Key words: Amino acid, Hydroxy iron oxide, Nano-assembly, Assembly kinetics, Biomaterial

CLC Number:

O635.1

TrendMD:

ZOU Haoyang, ZHAO Zhitong, WANG Huaizhi, YANG Jiazhen, DING Jianxun. Construction and Assembly Kinetics of Amino Acid-Iron Nano-assemblies[J]. Chem. J. Chinese Universities, 2025, 46(1): 20240385.

Figures/Tables 11

References 27

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Temperature/℃	10²k_Step1/（lnh）^-1	10²k_Step2/（lnh）^-1	10²k_Step3/（lnh）^-1
20	1.50	1.95	1.89
30	5.07	4.44	1.09
40	8.60	8.30	0.72
50	10.94	7.28	0.12

Temperature/℃	10²k_Step1/（lnh）^-1	10²k_Step2/（lnh）^-1	10²k_Step3/（lnh）^-1
20	1.50	1.95	1.89
30	5.07	4.44	1.09
40	8.60	8.30	0.72
50	10.94	7.28	0.12

Sample	Percentage of integral peak area（%）
Sample	20 ℃	30 ℃	40 ℃	50 ℃
β⁃FeOOH	27.4	24.7	32.6	34.7
β⁃FeOOH	28.1	28.6	26.5	30.1
Fe—COOH	24.0	24.9	21.1	18.8
Fe—NH₂	20.5	21.8	19.8	16.4

Sample	Percentage of integral peak area（%）
Sample	20 ℃	30 ℃	40 ℃	50 ℃
β⁃FeOOH	27.4	24.7	32.6	34.7
β⁃FeOOH	28.1	28.6	26.5	30.1
Fe—COOH	24.0	24.9	21.1	18.8
Fe—NH₂	20.5	21.8	19.8	16.4

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