Rapid Qualitative Analysis of Active Molecules in Tablets Using Microwave Plasma Torch Mass Spectrometry by the Regulation of Power†

doi:10.7503/cjcu20160686

Abstract

Abstract:

A microwave plasma torch mass spectrometry(MPT-MS) method was applied for the qualitative analysis of active ingredients in tinidazole and chlorpheniramine tablets. By changing the microwave power, both the parent ion and abundant fragment ions of the target were obtained in the full-scan mass spectra. These fragment ions were in accordance with the fragments from tandem mass spectrometry experiments, and even more abundant. Therefore, MPT source, with both hard ionization and soft ionization properties, could be used for the fast qualitative analysis of active ingredients in tablets, because it can obtain both parent and fragment ions in the full-scan mass spectrometry experiments. This rapid, accuracy, and no pollution MPT-MS method is useful for developing MPT coupled to a simple mass analyzer and realizing the miniaturization of mass spectrometer.

Key words: Microwave plasma torch, Mass spectrometry, Microwave power, Tablet

CLC Number:

O657.6

TrendMD:

WANG Xinchen, YANG Meiling, ZHANG Xiaoping. Rapid Qualitative Analysis of Active Molecules in Tablets Using Microwave Plasma Torch Mass Spectrometry by the Regulation of Power^†[J]. Chem. J. Chinese Universities, 2017, 38(4): 561.

Figures/Tables 8

Fig.1 Schematic of the MPT-MS setup

Fig.2 Full-scan mass spectra obtained by MPT-MS for tinidazole tablet(A, B) and tinidazole standard(C, D) at microwave powers of 30 W(A, C) and 130 W(B, D)

Fig.3 MS2 spectrum of tinidazole at m/z 248(A) and MS2 spectrum of chlorpheniramine at m/z 275(B)Insets of (A) are the MS3 spectra of m/z 248→m/z 202 and m/z 248→m/z 121); inset of (B) is the MS3 spectrum of m/z 275→230.

Fig.4 Proposed fragment pathways of [M+H]+ at m/z 248 for tinidazole

Table 1 Fragment ions of tinidazole and chlorpheniramine obtained by tandem MS

Tablet	Molecular weight	[M+H]⁺, m/z	MS², m/z	MS³, m/z
Tinidazole	247	248^a	202^b, 110	110
			128, 121^b	93
Chlorpheniramine	274	275^a	230^b	202, 167

Fig.5 Full-scan mass spectra obtained by MPT-MS for chlorpheniramine tablet(A, B) and chlorpheniramine standard(C, D) at microwave powers of 30 W(A, C) and 110 W(B, D)

Fig.6 Proposed fragmentation mechanism pathways of [M+H]+ at m/z 275 for chlorpheniramine

Fig.7 Effects of microwave power on the intensities of ions from active ingredients in tables by MPT-MS (A) Tinidazole; (B) chlorpheniramine.

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