SERS Nanoprobe for the Detection of Reactive Oxygen Species in Cells Produced by Electrostimulus

doi:10.7503/cjcu20220033

Abstract

Abstract:

Electrostimulus（ES） is an important therapeutic method for diseases caused by abnormal intracellular electrical activity. Also， ES can induce apoptosis of cells， which is a potential tumor treatment method. At present， there are no relevant studies on the intracellular reactive oxygen species（ROS） levels produced in the process of ES， and what effects will be brought by the simultaneous implementation of conventional antioxidant inhibitor drugs and ES therapy. In this study， a kind of gold/silver core-shell nanorod-based surface-enhanced Raman（SERS）-active probe with good biocompatibility was designed for the detection of intracellular ROS generated during ES. The sensing mechanism realized the quantitative analysis of ROS based on the principle that ROS could etch the silver shell， leading to a decrease of the SERS signal of the SERS probe. The probe was co-incubated with cells and was internalized into cells. The cells were then electrically stimulated for different periods of time. Finally， the signal of the SERS probe was detected in vivo. The experimental results show that the SERS signal weakens with the extension of ES time， indicating that the amount of ROS produced in cells increases significantly. In addition， two antioxidation inhibitors， ascorbic acid（AA） and glutathione（GSH）， were studied when combined with ES. It was observed that they could eliminate reactive oxygen species（ROS） produced by electrical stimulation. This study developed a new method for intracellular ROS detection and suggests a combination of drugs for abnormal oxidative stress and tumor therapy. Also， ES can induce apoptosis of cells， which is a potential tumor treatment method.

Key words: Reactive oxygen species, Surface-enhanced Raman spectroscopy, Electrostimulus, in situ Detection

CLC Number:

O657.37

TrendMD:

CHEN Jiamin, QU Xiaozhang, QI Guohua, XU Weiqing, JIN Yongdong, XU Shuping. SERS Nanoprobe for the Detection of Reactive Oxygen Species in Cells Produced by Electrostimulus[J]. Chem. J. Chinese Universities, 2022, 43(6): 20220033.

Figures/Tables 7

References 26

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