Investigation on the Enrichment of DNA Using Lanthanum Carbonate†

doi:10.7503/cjcu20150644

Abstract

Abstract:

The adsorption behavior of Deoxyribonucleic acid(DNA) on lanthanum carbonate particles was investigated. Both UV and XPS results demonstrated that DNA could be adsorbed on the surface of lanthanum carbonate particles. Moreover, the efficiency of adsorption was maximized when the pH value of the solution was set around 5.5. In addition, DNA could be desorpted when lanthanum carbonate particles with adsorpted DNA was dispersed in aqueous solution of KH₂PO₄. The ICP results demonstrated that the concentration of La³⁺ was lower than 0.1 μmol/L in the aqueous solution after desorption process, thereby excluding the possibility of undesirable influence on DNA.

Key words: Deoxyribonucleic acid(DNA), Lanthanum carbonate, Enrichment, Desorption

CLC Number:

O614.33
O652

TrendMD:

KANG Xiaoyan, HE Anqi, WANG Jingdan, GUO Ran, ZHAI Yanjun, XU Yizhuang, WU Jinguang. Investigation on the Enrichment of DNA Using Lanthanum Carbonate^†[J]. Chem. J. Chinese Universities, 2016, 37(1): 7.

Figures/Tables 7

Fig.1 Variation of the absorbance of the DNA peak at 260 nm as a function of the concentration of DNA

Fig.2 UV spectra of DNA solution before(a) and after(b) interacting with lanthanum carbonate particles

Fig.3 XPS spectra of lanthanum carbonate particles before(a) and after(b) interacting with aqueous solution of DNA

Fig.4 P2p XPS peak of lanthanum carbonate particles after interacting with aqueous solution of DNA^a. Test curve; b. peak 1; c. peak 2; d. baseline;e. fitting curve.

Fig.5 XRD patterns of lanthanum carbonate particles before(a) and after(b) interacting with aqueous solution of DNA

Fig.6 Variation of the adsorption efficiency of DNA on lanthanum carbonate particles as a function of pH value of the aqueous solution

Fig.7 UV spectrum of an aqueous solution of KH2PO4 after interacting with lanthanum carbonate particles with adsorped DNA

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