Investigation of Lipid Distributions by Trifluoroacetic Acid-enhanced Desorption Electrospray Ionization Mass Spectrometry Imaging

doi:10.7503/cjcu20200448

Abstract

Abstract:

A large number of lipids play an important role in the process of life， so it is important to simultaneously visualize multiple lipids by mass spectrometry imaging. By adding trifluoroacetic acid into the spray solvent of desorption electrospray ionization（DESI）， the number of detectable lipids was increased from 59 to 71 in the mouse brain tissue sagittal section. This method could not only improve the detection sensitivity， but also suppress the formation of sodium and potassium adducts of lipids， to simplify spectrum interpretation and facilitate quantitative analysis. Furthermore， the result also revealed the spatial correlation among the lipids in the whole mouse brain tissue section as well as specific brain regions. The present protocol provides a new research method for exploring potential metabolic pathways between lipids， suggesting the potential application in neuroscience， clinical， and other fields.

Key words: Lipid, Trifluoroacetic acid, Desorption electrospray ionization mass spectrometry imaging, Spatial correlation

CLC Number:

O657.63

TrendMD:

WANG Xiaoqun, HUANG Guangming. Investigation of Lipid Distributions by Trifluoroacetic Acid-enhanced Desorption Electrospray Ionization Mass Spectrometry Imaging[J]. Chem. J. Chinese Universities, 2020, 41(12): 2673.

Figures/Tables 6

References 29

1	Bosche B.， Schafer M.， Graf R.， Hartel F. V.， Schafer U.， Noll T.， Biochem. Biophys. Res. Commun.， 2013， 434， 268—272
2	Senadheera S.， Kim Y.， Grayson T. H.， Toemoe S.， Kochukov M. Y.， Abramowitz J.， Housley G. D.， Bertrand R. L.， Chadha P. S.， Bertrand P. P.， Murphy T. V.， Tare M.， Birnbaumer L.， Marrelli S. P.， Sandow S. L.， Cardiovasc. Res.， 2012， 95， 439—447
3	Ghesquiere B.， Wong B.W.， Kuchnio A.， Carmeliet P.， Nature， 2014， 511， 167—176
4	Di Paolo G.， Kim T. W.， Nat. Rev. Neurosci.， 2011， 12， 284—296
5	Herber D. L.， Cao W.， Nefedova Y. S.， Novitskiy V.， Nagaraj S.， Tyurin V.A.， Corzo A.， Cho H. I.， Celis E.， Lennox B.， Knight S. C.， Padhya T.， McCaffrey T. V.， McCaffrey J. C.， Antonia S.， Fishman M.， Ferris R. L.， Kagan V. E.， Gabrilovich D. I.， Nat. Med.， 2010， 16， 880—886
6	Takáts Z.， Wiseman J. M.， Gologan B.， Cooks R. G.， Science， 2004， 306， 471—473
7	Lostun D.， Perez C. J.， Licence P.， Barrett D. A.， Ifa D. R.， Anal. Chem.， 2015， 87， 3286—3293
8	Wiseman J. M.， Ifa D. R.， Song Q.， Cooks R. G.， Angew. Chem. Int. Ed.， 2006， 45， 7188—7192
9	Perry R. H.， Bellovin D. I.， Shroff E. H.， Ismail A. I.， Zabuawala T.， Felsher D. W.， Zare R. N.， Anal. Chem.， 2013， 85， 4259—4262
10	Wu C.， Ifa D. R.， Manicke N. E.， Cooks R. G.， Analyst， 2010， 135， 28—32
11	Wiseman J. M.， Puolitaival S. M.， Takáts Z.， Cooks R. G.， Caprioli R. M.， Angew. Chem. Int. Ed.， 2005， 44， 7094—7097
12	Manicke N. E.， Nefliu M.， Wu C.， Woods J. W.， Reiser V.， Hendrickson R. C.， Cooks R. G.， Anal. Chem.， 2009， 81， 8702—8707
13	Wang X.， Hou Y.， Hou Z.， Xiong W.， Huang G.， Anal. Chem.， 2019， 91， 2719—2726
14	Shrestha B.， Nemes P.， Nazarian J.， Hathout Y.， Hoffman E. P.， Vertes A.， Analyst， 2010， 135， 751—758
15	He J.， Sun C.， Li T.， Luo Z.， Huang L.， Song X.， Li X.， Abliz Z.， Adv. Sci.， 2018， 5， 1800250
16	Shrivas K.， Hayasaka T.， Sugiura Y.， Setou M.， Anal. Chem.， 2011， 83， 7283—7289
17	Shariatgorji M.， Nilsson A.， Goodwin R. J. A.， Svenningsson P.， Schintu N.， Banka Z.， Kladni L.， Hasko T.， Szabo A.， Andren P. E.， Anal. Chem.， 2012， 84， 7152—7157
18	Wiseman J. M.， Ifa D. R.， Venter A.， Cooks R. G.， Nature Protocols， 2008， 3， 517—524
19	Eberlin L. S.， Ferreira C. R.， Dill A. L.， Ifa D. R.， Cheng L.， Cooks R. G.， ChemBioChem， 2011， 12， 2129—2132
20	Eberlin L. S.， Ifa D. R.， Wu C.， Cooks R. G.， Angew. Chem. Int. Ed.， 2010， 49， 873—876
21	Nemes P.， Woods A. S.， Vertes A.， Anal. Chem.， 2010， 82， 982—988
22	Li Q.， Lau A.， Morris T. J.， Guo L.， Fordyce C. B.， Stanley E. F. J.， Neurosci.， 2004， 24， 4070—4081
23	McDonnell L. A.， van Remoortere A.， van Zeijl R. J. M.， Deelder A. M. J.， ProteomeRes.， 2008， 7， 3619—3627
24	Woo E. Y.， Chu C. S.， Goletz T. J.， Schlienger K.， Yeh H.， Coukos G.， Rubin S. C.， Kaiser L. R.， June C. H.， Cancer Res.， 2001， 61（12）， 4766—4772
25	Yang Z. Z.， Novak A. J.， Stenson M. J.， Witzig T. E.， Ansell S. M.， Blood， 2006， 107（9）， 3639—3646
26	Viguier M.， Lemaitre F.， Verola O.， Cho M. S.， Gorochov G.， Dubertret L.， Bachelez H.， Kourilsky P.， Ferradini L.， J. Immunol.， 2004， 173（2）， 1444—1453
27	Mcdaniel M. A.， Maier S. F.， Einstein G. O.， Nutrients， 2003， 19， 957—975
28	Hellhammer J.， Stress， 2004， 7（2）， 119—126
29	Palesi F.， Tournier J. D.， Calamante F.， Brain Sruct. Funct.， 2015， 220（6）， 3369—3384

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