Direct Analysis of Micro Area on Tooth Surface by Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry†

doi:10.7503/cjcu20150008

Abstract

Abstract:

A novel analytical platform based on a nanoliter needle for sampling and surface desorption atmospheric pressure chemistry ionization mass spectrometry(SDAPCI-MS) for analysis was developed for the direct, fast and micro-area analysis of different parts of a human tooth, including cavity, cavity edge, tooth cusp and tooth sulcus. The results showed that the mass spectra from the four sampling spots on the same tooth were different, and that SDAPCI-MS detected lactic acid, pyruvic acid, phenylacetic acid and propanoic acid were confirmed using tandem mass spectrometry(MS/MS). Principal component analysis(PCA) of the mass spectral data successfully differentiated the different sampling spots, levels of tooth decay and levels of gum disease. Therefore, our nano-SDAPCI-MS approach provides a promising way for micro-area analysis with fast, minimal pretreatment sampling, enabling rapid, simple and reliable surface MS investigation.

Key words: Surface desorption atmospheric pressure chemistry ionization, Mass spectrometry, Tooth disease, Micro-analysis

CLC Number:

O657

TrendMD:

WANG Jiang, LI Qian, GU Haiwei, GUO Xiaotun, YANG Shuiping, WANG Zhihao. Direct Analysis of Micro Area on Tooth Surface by Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry^†[J]. Chem. J. Chinese Universities, 2015, 36(6): 1067.

Figures/Tables 7

Fig.1 Schematic drawing of nano-SDAPCI-MS

Fig.2 SDAPCI-MS spectra of a cavity sample(A) and standard lactic acid(B) in negative ion detection mode Insets are MS/MS spectra of the peak at m/z 89(lactic acid).

Fig.3 Optimization of nano-SDAPCI-MS working conditions on the SDAPCI signal levels (A) Ionization voltage; (B) distance; (C) angle.

Fig.4 nano-SDAPCI-MS(A) and MS/MS(B—D) spectra for cavity samples in negative ion detection mode(B)—(D): MS/MS spectra of m/z 87, 135 and 73, respectively.

Fig.5 SDAPCI-MS spectra of an acupuncture needle for a blank sample(A), edge of cavity(B), tooth cusp(C) and tooth sulcus(D) from one tooth in negative ion detection mode

Fig.6 3D plots of PCA score results for cavity, edge of cavity, tooth cusp and tooth sulcus on the same tooth in negative ion detection mode

Fig.7 PCA results of dental disease in different degrees using SDAPCI-MS data in negative ion detection mode (A) 3D plots of PCA of caries-free, shallow caries, medium caries and deep caries; (B) 3D plots of PCA of healthy tooth, gingivitis, and periodontitis.

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