Chemical Journal of Chinese Universities ›› 2020, Vol. 41 ›› Issue (11): 2345-2355.doi: 10.7503/cjcu20200397

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Deoxyribozymes: Selection, Biosensing and Outlook

TONG Zongxuan, HU Qinqin, GU Hongzhou()   

  1. Shanghai Institute of Cardiovascular Diseases,and Institutes of Biomedical Sciences,Zhongshan Hospital,Fudan University,Shanghai 200032,China
  • Received:2020-06-29 Online:2020-11-10 Published:2020-11-06
  • Contact: GU Hongzhou
  • Supported by:
    ? Supported by the National Natural Science Foundation of China(21673050);the National Key Research and Development Program of China(2016YFC1306400)


In this review, we briefly describe the origin of deoxyribozymes and the selection strategies for the isolation of these deoxyribonucleic acid(DNA) enzymes. We compare deoxyribozymes with ribozymes and protein enzymes. We focus on the recently developed product-capturing and cancelling selection strategies, as well as the metal-ion-specific and particular-biological-sample-sensing ribonucleic acid(RNA)-cleaving deoxyribozymes which have worked as molecular tools in many aspects. In the end, we summarize the challenges for the deoxyribozyme field that need to be tackled down in the future.

Key words: Deoxyribozymes, Catalytic DNA, Systematic evolution of ligands by exponential enrichment(SELEX), Selection strategy

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