Fabrication and Characterization of PLGA-based Supermolecular Hydrogel†

doi:10.7503/cjcu20150551

Abstract

Abstract:

Supermolecular hydrogels composed of cholesterol-modified triblock polymers poly(L-glutamic acid)-poly(ethylene glycol)-poly(L-glutamic acid)[(PLGA-b-PEG-b-PLGA)-g-Chol] and β-cyclodextrins(β-CD)-modified poly(L-glutamic acid)(PLGA-g-β-CD) were fabricated by host-guest interaction between cholesterol(Chol) and β-CD. This study presented the influence of concentrations and molar ratio on mechanical strength, PLGA molecular weight on mechanical strength, pore size, swelling ratios and biodegradability. The results showed that storage modulus was highest at concentrations of 150 g/L and Chol/β-CD molar ratio of 1:1. The average pore size of PLGA-based supermolecular hydrogels fabricated from concentrations of 150 g/L was between 10—30 μm. Swelling ratio was between 16%—32.5%. Degradation time could be controlled PLGA molecular weight from 40 d to 72 d. The excellent self-healing ability of hydrogels was performed. The cytotoxicity evaluation was observed that the adipose-derived stem cells treated with the polymers remained almost 100% viable, indicating good cytocompatibility of the hydrogels.

Key words: Poly(L-glutamic acid), β-Cyclodextrin, Cholesterol, Supermolecular hydrogel, Storage modulus, Cytocompatibility

CLC Number:

O636

TrendMD:

LI Guifei, WU Jie, WANG Bo, ZHANG Weijun, YAN Shifeng, YIN Jingbo. Fabrication and Characterization of PLGA-based Supermolecular Hydrogel^†[J]. Chem. J. Chinese Universities, 2015, 36(12): 2582.

Figures/Tables 12

Scheme 1 Synthesis of PLGA-b-PEG-b-PLGA

Fig.1 1H NMR spectra of PLGA-b-PEG-b-PLGA a. PLGA1433-b-PEG2000-b-PLGA1433; b. PLGA13494-b-PEG2000-b-PLGA13494; c. PLGA30250-b-PEG2000-b-PLGA30250.

Scheme 2 Synthetic routes of (PLGA-b-PEG-b-PLGA)-g-Chol and PLGA-g-β-CD

Fig.2 1H NMR spectra of (PLGA-b-PEG-b-PLGA)-g-Chol(A) and PLGA-g-β-CD(B) (A) a. (PLGA1433-b-PEG2000-b-PLGA1433)-g-Chol19.67%; b. cholesterol; c. PLGA1433-b-PEG2000-b-PLGA1433;(B) a. PLGA-g-β-CD; b. β-CD-NH2·HCl; c. PLGA.

Scheme 3 Preparation process of supermolecular hydrogels

Fig.3 Photographs of PLGA1433, PLGA13494 and PLGA30250 hydrogels

Fig.4 Storage modulus(G', a—c) and loss modulus(G″, a'—c') of hydrogels PLGA1433 with concentrations of 50 g/L(a, a'), 100 g/L(b, b') and 150 g/L(c, c')(nChol/nβ-CD= 1:1)

Fig.5 Storage modulus(G', a—c) and loss modulus(G″, a'—c') of PLGA1433(a, a'), PLGA13494(b, b'), and PLGA30250(c, c') at concentration of 150 g/L

Fig.6 Storage modulus(G', a—c) and loss modulus(G″, a'—c') of hydrogels PLGA1433 (150 g/mL) with different ratios of β-CD and Chol nChol/nβ-CD: a, a'. 1:1; b, b'. 1:2; c, c'. 2:1.

Fig.7 SEM images of hydrogels PLGA1433(A), PLGA13494(B) and PLGA30250(C) at concentration of 150 g/L for nChol/nβ-CD=1:1

Fig.8 Swelling ratio(A) and degradation of hydrogels(B) for molar ratio of Chol/β-CD being 1:1 at concentration of 150 g/L

Fig.9 Cytotoxicity evaluation of the mixture solutions of(PLGA-b-PEG-b-PLGA)-g-Chol with PLGA-g-β-CD Data are presented as the average±standard deviation(n = 3).

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