Cell Response and Mechanical Properties of the Nanofibers Reinforced Bilayered Tissue Guided Memberanes†

doi:10.7503/cjcu20170336

Abstract

Abstract:

The bilayer guiding bone regeneration(GBR) membrane used for simultaneous periodontal regeneration was fabricated. Firstly, nano hydroxyapatite/polyamide(nHA/PA) membrane was prepared by thermally induced phase separation. Afterwards, PA nanofibers with different orientation was formed by electrospinning on the surface of the porous nano hydroxyapatite/polyamide(nHA/PA) membrane. The microstructure, orientation and mechanical properties of the PA nanofibers were investigated under different rotational speed(0, 500, 1500 r/min). The results show that porous structure is presented on the surface and inside of the nHA/PA membrane. The nHA crystals distribute uniformly in the PA matrix. The orientation of the PA nanofibers is improved with the increasing of rotation speed. The mechanical testing results show that the elastic strength of the bilayer construct can reach(39.86±4.73) MPa under the rotation speed of 500 r/min. The cell culture results show that the MG63 cells grow well around the bilayer construct. The bilayer constructs display favorable cytocompatibility and show potential clinical applications in the field of periodontal regeneration.

Key words: Electrospining, Bilayered tissue guided memberane, Mechanical property, Cytocompatibility, Polyamide nanofiber

CLC Number:

O646
O63

TrendMD:

WANG Zhiwei, HUANG Di, XI Shaohui, XU Mengjie, ZHU Xuefang, LIAN Xiaojie, WEI Yan, CHEN Weiyi. Cell Response and Mechanical Properties of the Nanofibers Reinforced Bilayered Tissue Guided Memberanes^†[J]. Chem. J. Chinese Universities, 2018, 39(1): 178.

Figures/Tables 7

Fig.1 SEM images of the surface(A, B) and the cross-section(C, D) of nHA/PA substrate with different magnificationsArrow in (D) indicates nHA crystals incorporated in PA matrix.

Fig.2 SEM images of bilayered constructs under different rotational speedsRotational speed/(r·min-1): (A) 0; (B) 500; (C) 1500. The dotted lines indicate the orientation of PA nanofibers.

Fig.3 SEM image of the interface of PA nanofibers and nHA/PA substrate

Fig.4 Stress and strain curves of nHA/PA substrate(A) and bilayered constructs under rotational speeds of 0(B), 500 r/min(C) and 1500 r/min(D)

Fig.5 SEM images of the fracture surface of samples with different magnifications(A—A’) nHA/PA substrate. Bilayered constructs under different rotational speed: (B—B’) 0 r/min; (C—C’) 500 r/min;(D—D') 1500 r/min. The dotted arrows indicate the direction of crack growth.

Fig.6 Phase contrast micrographs of MG63 cells cultured with nHA/PA substrate(A) and bilayered constructs(B) for 7 dM: material; C: cell.

Fig.7 MTT assays for proliferation of MG63 cells cultured with nHA/PA membranes and bilayered constructs for 1, 4, 7 d, compared with the blank control under the same culture conditionSignificantly different at *P<0.05.

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