Chem. J. Chinese Universities ›› 2014, Vol. 35 ›› Issue (9): 2019.doi: 10.7503/cjcu20140326
• Polymer Chemistry • Previous Articles Next Articles
HE Ning*(), SUN Hechun, XU Huanxi, SHAO Zhangzhang
Received:
2014-04-08
Online:
2014-09-10
Published:
2019-08-01
Contact:
HE Ning
E-mail:hening826@163.com
CLC Number:
TrendMD:
HE Ning, SUN Hechun, XU Huanxi, SHAO Zhangzhang. Synthesis and Biological Evaluation of Comblike Non-virus Gene Delivery Vector with the Poly(L-glutamic acid) as Backbone†[J]. Chem. J. Chinese Universities, 2014, 35(9): 2019.
Fig.1 1H NMR spectra of PBLG in CDCl3(A), PG-g-PEI(B) and PG-g-(PEI-b-PEG)(C) Peaks a—g are corresponding to the structures in Scheme 2, respectively.
Fig.4 Gel retardation assay of PG-g-(PEI-b-PEG)/pDNA complexes prepared at different N/P ratios Lane 1: free DNA; lanes 2—7: PG-g-(PEI-b-PEG)/pDNA complexes with N/P ratios of 0.5, 1, 3, 5, 10 and 20; lane 8: PG-g-(PEI-b-PEG) polymer solution.
Fig.7 Cell morphologies(A1—F1) and pEGFP-C1 expression under the observation of the fluorescence microscopies(A2—F2) using PG-g-(PEI-b-PEG) as the vector (A1, A2)—(F1, F2) are N/P ratio of 0.5, 3, 5, 10, 40 and 0, respectively.
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