Preparation of Cholesterol-graft-poly(D,L-lactic acid)/ Poly(D,L-lactic acid) Composite Nanofiber Membranes by Electrospinning

doi:10.3969/j.issn.0251-0790.2012.06.040

Abstract

Abstract: Cholesterol-graft-poly(D,L-lactic acid)(CHOL-g-PDLLA ) oligomer was synthesized by the ring-opening polymerization of D,L-lactide using stannous 2-ethylhexanoate as catalyst and cholesterol(CHOL) as co-initiator, and the liquid crystalline properties was observed by polarizing microscope and differential scanning calorimetry. Then, CHOL/PDLLA and CHOL-g-PDLLA/PDLLA composite nanofiber membranes were prepared by electrospinning. The micrograph, interfacial compatibility, porosity, tensile properties and cellular compatibility of the composite nanofiber membranes were studied. The results show that CHOL-g-PDLLA is a thermotropic cholesteric liquid crystal with liquid crystal temperature range of 21.8-74.5 ℃. CHOL-g-PDLLA/PDLLA composite nanofiber membranes with good fiber micrograph, smooth surface and porosity of 70% to 75% were obtained, and the interfacial compatibility was significantly better than that of CHOL/PDLLA. With the content of CHOL or CHOL-g-PDLLA increased, the tensile strength of CHOL/PDLLA and CHOL-g-PDLLA/PDLLA composite nanofiber membranes both decreased, but the tensile strength of the latter is always greater than that of the former. The results of mesenchymal stem cells culture in vitro indicate that the cellular compatibility of the CHOL-g-PDLLA/PDLLA composite nanofiber membranes is very good, and better than those of pure PDLLA and CHOL/PDLLA nanofiber membranes.

Key words: Electrospinning, Poly(D,L-lactic acid), Cholesterol-graft-poly(D,L-lactic acid), Liquid crystal, Nanofiber membrane

CLC Number:

O631

TrendMD:

LUO Bing-Hong, LI Jian-Hua, YANG Jing, XU Chong-En, TIAN Jin-Huan, ZHOU Chang-Ren. Preparation of Cholesterol-graft-poly(D,L-lactic acid)/ Poly(D,L-lactic acid) Composite Nanofiber Membranes by Electrospinning[J]. Chem. J. Chinese Universities, 2012, 33(06): 1360.

References

[1] Deng M., Nair L. S., Nukavarapu S. P., Kumbar S. G., Brown J. L., Krogman N. R., Weikel A. L., Allcock H. R., Laurencin C. T.. J. Biomed. Mater. Res.[J], 2010, 92A(1): 114—125 [2] LIU Shu-Qiong(刘淑琼), XIAO Xiu-Feng(肖秀峰), LIU Rong-Fang(刘榕芳), LIN Yue-Hong(林月红), ZHONG Zhang-Yu(钟章裕), JIAO Jian-Jin(焦简金). Chem. J. Chinese Universities(高等学校化学学报)[J], 2011, 32(2): 372—378 [3] Parrish B., Breitenkamp R. B., Emrick T.. J. Am. Chem. Soc.[J], 2005, 127(20): 7404—7410 [4] Liu X., Li K. R., Feng X. L., Cao Y.. J. Macromol. Sci., Pure Appl. Chem.[J], 2009, 46(10): 937—942 [5] Parrish B., Emrick T.. Macromolecules[J], 2004, 37(16): 5863—5865 [6] Eliska T., Stepan P., Ludka M., Frantisek R.. Biomacromolecules[J], 2010, 11(1): 68—75 [7] YANG Fu-Hua(杨抚华). Medical Cell Biology(医学细胞生物学)[M], Beijing: Science Press, 2010: 40-48 [8] Yu G. H., Ji J., Shen J. C.. J. Mater. Sci.: Mater. Med.[J], 2006, 17: 899—909 [9] YU Guan-Hua(余贯华), JI Jian(计剑), SHEN Jia-Cong(沈家骢). Acta Polymerica Sinica(高分子学报)[J], 2006, (1): 136—140 [10] Dahl J. S., Dahl C. E., Bloch K.. J. Biol. Chem.[J], 1981, 256(1): 87—91 [11] Jana1R. N., Cho J. W.. Fibers and Polymers[J], 2009, 10(5): 569—575 [12] ZHAO Jin(赵瑾), ZHAO Yu-Ping(赵玉平), ZHANG Wei(张伟), YUAN Xiao-Yan(袁晓燕). Chem. J. Chinese Universities(高等学校化学学报)[J], 2009, 30(2): 391—395 [13] Ladd M. R., Lee S. J., Stitzel J. D., Atala A., Yoo J. J.. Biomaterials[J], 2011, 32(6): 1549—1559 [14] Gupta D., Venugopal J., Mitra S., Dev V. R. G., Ramakrishna S.. Biomaterials[J], 2009, 30: 2085—2094 [15] Cheng M. L., Chen P. Y., Lan C. H., Sun Y. M.. Polymer[J], 2011, 52(6): 1391—1401 [16] Klok H. A., Hwang J. J., Iyer S. N., Stupp S. I.. Macromolecules[J], 2002, 35(3): 746—759