Preparation and Enrichment Properties of Novel Magnetic Restricted Access Media-molecularly Imprinted Composites†

doi:10.7503/cjcu20180772

Abstract

Abstract:

The magnetic attapulgite(MATP) was preparrd by pyrolysis method. Then, using sulfadimethoxine(SDM) as template molecule, 4-vinylpyridine(4-VP) as functional monomer, the imprinted layer was coated on the surface of modified MATP via reverse atom transfer radical polymerization(RATRP), getting magnetic molecularly imprinted polymers(MATP-MIPs). Finally, novel magnetic restricted access media-molecularly imprinted composites(MATP-RAM-MIPs) was obtained by grafting hydrophilic monomer hydroxyethyl methacrylate(HEMA) onto the surface of MATP-MIPs. The polymer was characterized by means of Fourier transform infrared(FTIR) spectrum, scanning electron microscope(SEM), thermogravimetric analysis(TGA), vibrating sample magnetometer(VSM) and X-ray diffraction(XRD). The hydrophilicity of MATP-RAM-MIPs and the ability of exclusion of proteins were studied by the water contact angle and Coomassie brilliant blue G-250 method. The adsorption characters of MATP-RAM-MIPs on SDM were studied by adsorption isothermal and selectivity in detail, and MATP-RAM-MIPs were used as solid phase extraction packing to separate and enrichment of SAs from bovine samples. The spiked recoveries were 86.2%—100.6%, and the relative standard deviations(RSD) were 2.1%—4.2%, indicating that this material has application value in the pretreatment of complex samples.

Key words: Magnetic attapulgite, Reverse atom transfer radical polymerization, Restricted access mediamolecularly imprinted polymer, Adsorption property

CLC Number:

O657

TrendMD:

WU Shanshan,WEI Chanling,ZHAO Lijuan,TIAN Yang,WANG Xia,GONG Bolin. Preparation and Enrichment Properties of Novel Magnetic Restricted Access Media-molecularly Imprinted Composites^†[J]. Chem. J. Chinese Universities, 2019, 40(6): 1150.

Figures/Tables 10

Fig.1 Synthetic procedure of MATP-RAM-MIPs

Fig.2 FTIR spectra of ATP(a), MATP(b), KHMATP(c),MATP-MIPs(d) and MATP-RAM-MIPs(e)

Fig.3 SEM images of ATP(A), KHATP(B), MATP-MIPs(C) and MATP-RAM-MIPs(D)

Fig.4 Water contact angle profiles of MATP-RAM-MIPs(A) and MATP-MIPs(B)

Fig.5 MATP-RAM-MIPs(a), MATP-MIPs(b), MATP-RAM-NIPs(c) and MATP-NIPs(d) absorption of bovine serum albumin by coomassic brilliant blue G-250 method(A) and UV-Vis absorption spectra(B) of SDM(a), BSA(b) and MATP-RAM-MIPs-SPE(c)

Fig.6 Adsorption isotherm curves of MATP-RAM-MIPs(a) and MATP-RAM-NIPs(b)

Table 1 Comparison of adsorption capacities of various adsorbents

Solid support	Preparation method	Analyte	Adsorption capacity/(mg·g^-1)	Ref.
Polymer particles	Ultraviolet initiation for bulk polymerization	Sulfadiazine	6.73	[22]
Magnetic polymer microspheres	Ultraviolet initiation for bulk polymerization	Sulfadiazine	2.24	[23]
Molecularly imprinted column	Ultraviolet initiation for bulk polymerization	Sulfadiazine	0.87	[24]
Graphene oxide	Precipitation polymerization	Sulfadimethoxine	8.69	[25]
Magnetic nanoparticles	Surface grafting polymerization	Sulfadimethoxine	0.16	[26]
Polymeric substance	Silica gel as sacrificial materials	Sulfadimethoxine	0.31	[27]
Magnetic attapulgite	Reverse atom transfer radical polymerization	Sulfadimethoxine	8.79	This work

Fig.7 Selective absorption of MATP-RAM-MIPs and MATP-RAM-NIPs on SDM, SMR and SIZ

Fig.8 Chromatograms of bovine samples by HPLC a. Elution solutions of MATP-RAM-MIPs; b. elution solutions of MATP-RAM-NIPs; c. elution solutions of MATP-MIPs; d. elution solutions of MATP-MIPs; e. blank bovine sample. Spiked level: 50 μg/mL.

Table 2 Spiked recoveries and relative standard deviations(RSDs) of the SDM, SMR and SIZ in bovine samples(n=5)

Added substance	Added concentration/(μg·mL^-1)	Recovery(%)	RSD(%)
Sulfadimethoxine	25	93.7	3.7
	50	100.6	2.1
	100	99.3	3.0
Sulfamerazine	25	91.5	4.2
	50	87.3	2.5
	100	95.0	3.9
Sulfisoxazole	25	86.2	3.4
	50	92.8	2.6
	100	89.1	3.3

References 28

[1]	Chen X. J., Liu G. Q., Ren C. R., Gao M. J., Fan X. D., Chem. J. Chinese Universities,2018, 39(2), 212-218
	(陈小娟, 刘根起, 任宸锐, 高敏君, 范晓东. 高等学校化学学报, 2018, 39#(2), 212-218)
[2]	Segura P. A., Tremblay P., Picard P., Gagnon C., Sauvé S., J. Agric. Food Chem.,2010, 58(3), 1442-1446
[3]	Guo X. T., Yin Y. Y., Yang C., Qian Z., Res. Chem. Intermed.,2016, 42(7), 6423-6435
[4]	Li G. J., Liu C., Wang D. W., Ding L., Chem. Res. Chinese Universities,2018, 34(6), 893-898
[5]	Nováková L.,Vléková H., Anal. Chim.Acta,2009, 656(1/2), 8-35
[6]	He J., Song L., Chen S., Li Y., Wei H., Zhao D., Gu K., Zhang S., Food Chemistry,2015, 187, 331-337
[7]	Henrique F. B., Ribeiro S. M., Nascimento A. F., Mauro L. F., Trac-Trend Anal. Chem.,2015, 71(1), 9-25
[8]	Zhang X. A., Lin W. F., Chen S. F., Xu H., Gu H. C., Langmuir,2011, 27(22), 13669-13674
[9]	Ge H., Huang H. L., Xu M., Chem. J. Chinese Universities,2016, 37(8), 1551-1558
	(葛昊,黄海龙, 徐敏.高等学校化学学报, 2016, 37(8), 1551-1558)
[10]	Li X. Q., Yang Z., Zhang Q. H., Li H. M., Anal. Chim. Acta,2014, 807, 75-83
[11]	Ma F. C., Li X., Ren X. H., Chem. J. Chinese Universities,2017, 38(8), 1347-1353
	(马芳晨,李欣,任晓慧. 高等学校化学学报, 2017, 38(8), 1347-1353)
[12]	Murielle G., Marie-Pierre C., Hurtaud-Pessel D., Verdon E., J. Chromatography B,2016, 1011, 145-150
[13]	Zhao R., Li X., Sun B. L., Li Y. M., Li Y. Z., Wang C., Chem. Res. Chinese Universities,2017, 33(6), 986-994
[14]	Souza I. D., Melo L. P., Jardim I. C., Monteiro J. C., Nakano A. M., Queiroz M. E., Anal. Chim. Acta,2016, 932, 49-59
[15]	Du B., Qu T. T., Chen Z., Cao X. H., Han S. P., Shen G. P., Wang L., Talanta,2014, 129, 465-472
[16]	Wang J., Cormack P. A. G., Sherrington D. C., Angew. Chem. Int. Ed.,2003, 42(43), 5336-5338
[17]	Elijan A., Qiang F., Qi F., Rong L., Aiping X., Aiguo Z., Chun C., J. Polym. Res.,2010, 17, 401-409
[18]	Li Y., Li X., Dong C. K., Carbon,2010, 48, 3427-3433
[19]	Yue G. Q., Liu C., Wang D. Z., Mater. Res. Bull.,2010, 45, 1319-1323
[20]	Wang J. S., Matyjaszewski K., Macromolecule,1995, 28, 7572-7575
[21]	Gao D. G., Zhang Y. H., Ma J. Z., Material Review A,2017, 31(11), 123-129
	(高党鸽,张亚红,马建中. 材料导报A, 2017, 31(11), 123-129)
[22]	Du B. B., Xu Y., Huang Y. H., Kang M., Chinese J. Anal. Chem.,2012, 40(12), 1871-1876
	(杜碧柏,许杨,黄运红,康敏. 分析化学, 2012, 40(7), 1871-1876)
[23]	Hu Y. F., Wang C., Li X. D., Liu L. F., Electrophoresis,2017, 38(19), 2462-2467
[24]	Huang Y. H., Xu Y., Du B. B., He Q. H., Cao Y. S., Chinese J. Anal. Chem.,2012, 40(7), 1011-1018
	(黄运红,许杨,杜碧柏,何庆华,曹郁生. 分析化学, 2012, 40(7), 1011-1018)
[25]	Wei X. B., Xing X., Wen Q., Wu Y., Wang L. H., Prog. Nat. Sci.,2017, 27(3), 374-379
[26]	Kong X., Gao R. X., He X. W., Chen L. X., Zhang Y. K., J. Chromatography A,2012, 1245, 8-16
[27]	Liu H. J., Xiang W. Z., Xu W. J., Xiong Y. Q., Lu Y. B., Chinese J. Appl. Chem.,2005, 22(1), 83-86
	(刘慧君,项伟中,徐伟箭,熊远钦,卢彦兵. 应用化学, 2005, 22(1), 83-86)
[28]	Xu Z. G., Du Z., Hu Y. L., Hu Y. F., Pan Y. P., Li G. K., Chinese J. Anal. Chem.,2012, 40(7), 1002-1010
	(许志刚,杜卓,胡玉玲,胡玉斐,潘英朋,李攻科. 分析化学, 2012, 40(7), 1002-1010)