Structural Characterization of an Immunoactive Polysaccharide DNP-W1B from Dendrobium nobile Lindl.

doi:10.7503/cjcu20120883

Abstract

Abstract:

Dendrobium nobile Lindl.(Chinese name "Jin-Chai-Shi-Hu") is a species of Orchidaceae with distribution in Southeast Asia, southwest and south of China. In this work, a water-soluble heteropolysaccharide DNP-W1B with a molecular mass of 7.7×10⁵ and specific rotation of ²⁰_D=+81.3°(c 0.3, H₂O) was isolated from the stems of D. nobile Lindl. Monosaccharide composition analysis showed that DNP-W1B contained glucose, arabinose and galactose in a molar ratio of 6.2: 3.1: 0.9. The structural features of DNP-W1B were revealed by the combination of chemical and instrumental analysis, including IR, GC, methylation analysis and NMR. The results showed that DNP-W1B possessed a backbone of a disaccharide unit of with 33% branches at O-4 of (1→6)-linked β-D-glucopyranosyl residues. The side chains contained arabinosyl and galactosyl chains. Primary immunological tests in vitro indicated that it could stimulate LPS-induced B lymphocyte and Con A-induced T lymphocyte proliferation. Survey showed that DNP-W1B was a novel immunoactive polysaccharide compound from Dendrobium Sw.

Key words: Dendrobium nobile Lindl., Polysaccharide, Structure analysis, Immunological activity

CLC Number:

O629

TrendMD:

WANG Jun-Hui, ZHA Xue-Qiang, PAN Li-Hua, LUO Jian-Ping. Structural Characterization of an Immunoactive Polysaccharide DNP-W1B from Dendrobium nobile Lindl.[J]. Chem. J. Chinese Universities, 2013, 34(4): 881.

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