Detection of a Novel Metabolite of Urea in Soil by Extractive Electrospray Ionization Mass Spectrometry

doi:10.7503/cjcu20120814

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (6): 1379.doi: 10.7503/cjcu20120814

• Analytical Chemistry • Previous Articles Next Articles

Detection of a Novel Metabolite of Urea in Soil by Extractive Electrospray Ionization Mass Spectrometry

PAN Su-Su¹, ZHAO Na², OUYANG Yong-Zhong¹, HUANG Ke-Ke³, DING Jian-Hua¹, CHEN Huan-Wen¹, YUAN Long³, WANG Xing-Xiang⁴

1. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang 330013, China;
2. Jiangxi Bureau of Surveying and Mapping, Nanchang 330025, China;
3. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, China;
4. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China

Received:2012-09-04 Online:2013-06-10 Published:2013-04-16

Abstract

Abstract:

As an important nitrogen(N) source for the growth of many organisms, urea is intensively used as a nitrogen fertilizer. A better understanding of urea metabolism is required. Using extractive electrospray ionization tandem mass spectrometry(EESI-MSⁿ), a new urea metabolite(3,3-diamino-3-hydroxypropanoic acid) was detected in soil extracts from the rhizosphere of Pinus massoniana. A 3,3-diamino-3-hydroxypropanoic acid standard was synthesized from urea, sodium acetate and lithium tert-butoxide. The protonated molecular ion and the sodium adduct were observed in the mass spectrum. With tandem mass spectrometry, the two signals from the synthetic sample were quickly identified and verified the protonated molecular ion from the soil extracts. It can be inferred that 3,3-diamino-3-hydroxypropanoic acid is a metabolite from urea in soil, and this study provides clues for future research of nitrogen transformation processes in urea fertilized soils. EESI-MS is a promising tool for the rapid and accurate identification of trace levels of short-lived metabolites.

Key words: Extractive electrospray ionization, Tandem mass spectrometry, Soil, Urea metabolite

CLC Number:

O657.63

TrendMD:

PAN Su-Su, ZHAO Na, OUYANG Yong-Zhong, HUANG Ke-Ke, DING Jian-Hua, CHEN Huan-Wen, YUAN Long, WANG Xing-Xiang. Detection of a Novel Metabolite of Urea in Soil by Extractive Electrospray Ionization Mass Spectrometry[J]. Chem. J. Chinese Universities, 2013, 34(6): 1379.

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Detection of a Novel Metabolite of Urea in Soil by Extractive Electrospray Ionization Mass Spectrometry

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