Interaction of 6-Thioguanine with Cucurbit[7]uril and Bovine Serum Albumin by Spectroscopic Method

doi:10.7503/cjcu20120645

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (2): 375.doi: 10.7503/cjcu20120645

• Organic Chemistry • Previous Articles Next Articles

Interaction of 6-Thioguanine with Cucurbit[7]uril and Bovine Serum Albumin by Spectroscopic Method

HUANG Ying¹, WANG Juan², GUO Gai-Ying², TAO Zhu², XUE Sai-Feng², ZHU Qian-Jiang², ZHOU Qing-Di³

1. The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources, Ministry of Education;
2. Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang 550025, China;
3. School of Chemistry, the University of Sydney, NSW 2006, Australia

Received:2012-07-06 Online:2013-02-10 Published:2013-01-21

Abstract

Abstract:

The aim of the present study was to investigate the interaction of 6-thioguanine(6-TG) with cucurbit[7]uril(Q[7]) and bovine serum albumin(BSA) in an acetate buffer solution by ultraviolet absorption spectroscopy, fluorescence emission spectroscopy and synchronous fluorescence spectra, as well as and three-dimensional fluorescence spectra. It was indicated from UV-Vis spectra that 6-TG-Q[7] binary complex formed. The fluorescence quenching of BSA by 6-TG was a result of the formation of 6-TG-BSA complex, and the quenching mechanism of BSA by 6-TG was static quenching mechanism. Moreover, effect of 6-TG on BSA conformation was discussed by the studies of synchronous and three-dimensional fluorescence spectra. In addition, a specific binding interaction took place between the 6-TG and Q[7] in the presence of BSA.

Key words: 6-Thioguanine, Cucurbit[7]uril, Bovine serum albumin, Ultraviolet absorption spectroscopy, Fluorescence spectroscopy

CLC Number:

O625

TrendMD:

HUANG Ying, WANG Juan, GUO Gai-Ying, TAO Zhu, XUE Sai-Feng, ZHU Qian-Jiang, ZHOU Qing-Di. Interaction of 6-Thioguanine with Cucurbit[7]uril and Bovine Serum Albumin by Spectroscopic Method[J]. Chem. J. Chinese Universities, 2013, 34(2): 375.

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Interaction of 6-Thioguanine with Cucurbit[7]uril and Bovine Serum Albumin by Spectroscopic Method

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