高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

水溶性量子点纳米微球的制备、表征及其在生物检测中的应用

李德娜1, 张兵波1, 马贵平2, 刘旭辉2, 田惠1, 张泰平1, 常津1   

    1. 天津大学材料科学与工程学院纳米生物技术研究所, 天津 300072;
    2. 北京出入境检疫检验局技术中心, 北京 100029
  • 收稿日期:2007-05-23 修回日期:1900-01-01 出版日期:2008-01-10 发布日期:2008-01-10
  • 通讯作者: 常津

Preparation and Characterization of Hydrophilic Quantum Dot-tagged Nanoparticles and Their Application in Biodetection

LI De-Na1, ZHANG Bing-Bo1, MA Gui-Ping2, LIU Xu-Hui2, TIAN Hui1, ZHANG Tai-Ping1, CHANG Jin1*   

    1. Institute of Nanobiotechnology, School of Materials Science & Engineering, Tianjin University, Tianjin 300072, China;
    2. Center of Technology, Beijing Entr-exit Inspection and Quarantine Bureau, Beijing 100029, China
  • Received:2007-05-23 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
  • Contact: CHANG Jin

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被引次数

30

摘要： 通过超声乳化(O/W)法, 在CdSe/CdS荧光量子点外包覆一层双亲性高分子外壳, 制得水溶性量子点纳米微球. 用荧光发射光谱(PL)和透射电子显微镜(TEM)等手段对产品进行了表征. 结果表明, 此种方法简单易行, 制得的量子点纳米微球(70 nm)具有良好的水溶性、稳定性以及较强的荧光发射强度. 用这种改性后的量子点标记的免疫球蛋白分子能够识别专一抗原, 因此这种纳米粒子将有望进一步应用于生物检测.

关键词: 量子点, 双亲性高分子, 超生乳化, 生物检测

Abstract: Hydrophilic nanoparticles for biological assay were prepared by encapsulating luminescent quantum dots into an amphiphilic polymer via the method of ultrasonic emulsification(O/W). The samples were characterized via photoluminescence(PL) and transmission electron microscope(TEM). The results indicated that the method was simple, time/energy-saving and environmentally friendly. QDs-tagged nanoparticles obtained by this procedure were water-soluble, highly stable and bright in fluorescence emission with a size of 70 nm. After being water-solubilized by amphiphilic polymer, the QDs were labeled by IgGs and used in the biodetection. Spectral analysis showed that on FRET appeared in the water-soluble QDs modified by amphiphilic polymer. The immunofluorescence in vitro indicated that the QDs-IgGs bioconjugates had excellent specific bingding detection ability with nearly non-specific binding. On the basis of the properties QDs-tagged nanoparticles may have great potential for biodetection.

Key words: Quantum dots(QDs), Amphiphilic polymer, Ultrasonic emulsification, Biodetection

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