高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (8): 1373.

• 研究快报 • 上一篇    下一篇

血红蛋白与NO分子间相互作用的电化学表征

李清文, 高宏, 黄昊, 王义明, 冯军, 罗国安   

  1. 清华大学化学系, 北京 100084
  • 收稿日期:2000-08-26 出版日期:2001-08-24 发布日期:2001-08-24
  • 通讯作者: 罗国安(1946年出生),男,教授,博士生导师,从事生命分析化学研究.
  • 基金资助:

    国家九七三项目(批准号:G1999054404);国家自然科学基金重点项目(批准号:20075015)

Electrochemical Characterization on the Interaction Between Hemoglobin and NO Molecule

LI Qing-Wen, GAO Hong, HUANG Hao, WANG Yi-Ming, FENG Jun, LUO Guo-An   

  1. Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2000-08-26 Online:2001-08-24 Published:2001-08-24

PDF (PC)

被引次数

55

关键词: 血红蛋白, NO, 相互作用, 电化学方法, 纳米TiO2膜

Abstract: Nanocrystalline TiO2 particles film has been considered as an eminent matrix for the immobilization of biomolecules due to its high biocompatiability, high protein loading and special binding ability. The direct electron transfer process of hemoglobin was achieved at nanosized TiO2 film electrode, in which a well-defined redox peak of hemoglobin could be observed. However, this peak decreased with the presence of NO, indicating the electrochemical activity of hemoglobin was inhibited by the interation between hemoglobin and NO. While the further evidence revealed that such activity could be recovered after releasing NOfrom hemoglobin with electrochemical oxidation method.

Key words: Hemoglobin, NO, Interaction, Electrochemical method, Nanocrystalline, TiO2-film

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