高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (12): 1862.

• 研究简报 • 上一篇    下一篇

增强型电荷耦合器件用于毛细管电泳激光诱导荧光的检测

马明生, 吴晓军, 刘国诠   

  1. 中国科学院化学研究所, 北京, 100080
  • 收稿日期:1996-01-04 出版日期:1996-12-24 发布日期:1996-12-24
  • 通讯作者: 刘国诠
  • 作者简介:马明生,男,33岁,博士后.
  • 基金资助:

    国家教育委员会博士后基金

Laser-Induced Fluorcence-Intensified Charge Coupled-Device(LIF-ICCD) Used for Detection of Capillary Electrophoresis

MA Ming-Sheng, WU Xiao-Jun, LIU Guo-Quan   

  1. Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080
  • Received:1996-01-04 Online:1996-12-24 Published:1996-12-24

关键词: 增强型电荷耦合器件, 激光诱导荧光, 毛细管电泳, 异硫氰酸荧光黄

Abstract: Laser-induced fluorescence-intensified charge coupled-device(LIF-ICCD)was applied as detection of capillary electrophoresis.Various effects were studied both for the data collection of frequency and sensitivity of fluorescence detection in order to arrive at the optimun conditions.Acapillary electrophoregram of 4.5 ×10-12 mol/Lof fluorescein isothiocyanate(FITC)was obtained with the LIF-ICCD detector under the chosen conditions.

Key words: ICCD detector, Laser-induced fluorescence, Capillary electrophoresis, Fluorescein isothiocyanate(FITC)

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