高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (12): 1859.

• 研究简报 • 上一篇    下一篇

基于固定化酶的化学发光停流法测定D-氨基酸

封满良, 黄玉文, 宫志龙, 章竹君   

  1. 陕西师范大学化学系, 西安, 710062
  • 收稿日期:1995-12-13 出版日期:1996-12-24 发布日期:1996-12-24
  • 通讯作者: 章竹君
  • 作者简介:封满良,男,32岁,讲师.

Chemiluminescence Determination of D-amino Acid Based on Immobilized Enzymes

FENG Man-Liang, HUANG Yu-Wen, GONG Zhi-Long, ZHANG Zhu-Jun   

  1. Department of Chemistry, Shaanxi Nornzal University, Xi'an, 710062
  • Received:1995-12-13 Online:1996-12-24 Published:1996-12-24

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关键词: 固定化酶, 化学发光, 甲壳质, D-氨基酸

Abstract: D-amino acid oxidase and HRPwere separately immobilized on chitosan adsorbed on the silica gels to develop a chemiluminescent method,which was combined with two oxidation reactions of D-amino acid producing H2O2 catalyzed by D-amino acid oxidase and of Luminol-H2O2 generating CLcatalyzed by HRPtogether,also was stopped──flow technique used in this system. It has a good selectivity and sensitivity. Under optimized conditions,the detection limit is 1.8 ×10-5g/mL,the liner range of D-amino acid concentration was from 8× 10-8 to 1. 4×10-6g/mL,It was practicable to determine D-amino acid in serum directly with satisfactory results.

Key words: Immobilized enzymes, CL, Chitosan, D-amino acid

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