高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (4): 638.

• 研究简报 • 上一篇    下一篇

采用高效液相色谱技术分析生物体内维生素B6

张剑韵1, 黄龙全1, 早川享志2, 柘植治人2   

  1. 1. 安徽农业大学蚕业丝绸系, 合肥 230036;
    2. 岐阜大学农学部食品科学系, 日本岐阜 50121193
  • 收稿日期:2003-04-22 出版日期:2004-04-24 发布日期:2004-04-24
  • 通讯作者: 张剑韵(1959年出生),女,博士,副教授,从事昆虫生理生物化学和维生素学研究.E-mail:jianyun218@yahoo.com.cn E-mail:jianyun218@yahoo.com.cn
  • 基金资助:

    教育部留学回国人员科学研究启动基金(2002年)资助

Analysis of Vitamin B6 Derivatives in Biological Samples with High Performance Liquid Chromatography

ZHANG Jian-Yun1, HUANG Long-Quan1, HAYAKAWA Takashi2, TSUGE Haruhito2   

  1. 1. Department of Sericulture and Silk, Anhui Agricultural University, Hefei 230036, China;
    2. Department of Food Science, Faculty of Agriculture, Gifu University, Gifu 501-1193, Japan
  • Received:2003-04-22 Online:2004-04-24 Published:2004-04-24

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关键词: 高效液相色谱, KCN处理, 荧光检测, 维生素B6

Abstract: For the determination of vitamin B6 derivatives, a high performance liquid chromatography(HPLC) system composed of a single reversed-phase octadecylsilane column and fluorescence detector with an isocratic elution was proposed. This HPLC system perfectly analyzed pyridoxine, pyridoxal, pyridoxamine, pyridoxine 5′-phosphate and pyridoxamine 5′-phosphate together with pyridoxic acid in biological samples. In this method, relatively low fluorescence intensity of pyridoxal 5′-phasphate was improved by converting it to pyridoxic 5′-phosphate with KCN. The analytical procedure is very simple and economical, it is a useful tool for studying vitamin B6 in animal body.

Key words: High performance liquid chromatography(HPLC), KCN treatment, Fluorescence detection, Vitamin B6

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