高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (8): 1614-1620.doi: 10.7503/cjcu20190055

• 分析化学 • 上一篇    下一篇

基于UPLC-Q-Orbitrap MS/MS技术研究酸枣仁发酵过程中的化学成分转化

杜晨晖1, 李泽1, 崔小芳1, 张敏2, 裴香萍1, 詹海仙1, 闫艳2   

  1. 1. 山西中医药大学中药学院, 太原 030619;
    2. 山西大学中医药现代研究中心, 太原 030006
  • 收稿日期:2019-01-18 修回日期:2019-07-23 出版日期:2019-08-10 发布日期:2019-07-12
  • 通讯作者: 闫艳,女,博士,副教授,主要从事中药体内过程研究及中药质量控制方面的研究.E-mail:yanyan520@sxu.edu.cn E-mail:yanyan520@sxu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号:81603251,81603289)、山西省科技厅中药现代化关键技术研究振东专项(批准号:2014ZD0306,2016ZD0105,2016ZD0104)、山西省自然科学基金(批准号:2015011105)、晋药综合开发利用协同创新中心项目(批准号:2017-JYXT-25)和山西中医药大学科技创新团队项目(批准号:2018-TD-009)资助.

Chemical Transformation of Raw and Fermented Ziziphi Spinosae Semen Analyzed by UPLC-Q-Orbitrap MS/MS

DU Chenhui1, LI Ze1, CUI Xiaofang1, ZHANG Min2, PEI Xiangping1, ZHAN Haixian1, YAN Yan2   

  1. 1. School of Traditional Chinese Materia Medica, Shanxi University of Chinese Medicine, Taiyuan 030619, China;
    2. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
  • Received:2019-01-18 Revised:2019-07-23 Online:2019-08-10 Published:2019-07-12
  • Supported by:
    Supported by the National Natural Science Foundation of China(Nos.81603251, 81603289), the Zhendong Group Research Foundation of Shanxi Province, China(Nos.2014ZD0306, 2016ZD0105, 2016ZD0104), the Natural Science Foundation of Shanxi Province, China(No.2015011105), the Shanxi Collaborative Innovation Center for Comprehensive Development and Utilization of Jinyao Foundation, China(No.2017-JYXT-25) and the Science and Technology Innovation Group of Shanxi University of Chinese Medicine, China(No.2018-TD-009).

摘要: 利用超高效液相色谱-四极杆-静电场轨道阱串联质谱(UPLC-Q-Orbitrap MS/MS)研究了酸枣仁经茯苓发酵前后的主要化学成分变化,初步探讨了茯苓对酸枣仁发酵的转化规律.鉴定了发酵产物中24种化学成分,整合多元统计方法对酸枣仁发酵0,7和14 d后的差异性化学成分进行研究,采用正交偏最小二乘分析找到7个差异成分.采用t-检验对24种化学成分的相对含量进行分析;并分析了酸枣仁中黄酮苷、皂苷类成分的代谢反应途径.通过茯苓发酵,酸枣仁中化学成分主要发生了脱酰基和脱糖基等水解反应.为酸枣仁发酵产物的化学成分检测提供了快速、直观且准确的方法,为酸枣仁-茯苓共发酵产物的深入开发与利用提供了科学依据.

关键词: 酸枣仁, 茯苓, 发酵, 超高效液相色谱-四极杆-静电场轨道阱串联质谱, 生物转化

Abstract: To elucidate the fermentation-induced chemical transformation mechanisms of secondary metabolites, the chemical difference between raw and fermented Ziziphi Spinosae Semen(SZR) using Poria cocos (Schw.) Wolf was revealed by integrating UPLC-Q-Orbitrap MS/MS techniques-based untargeted metabolomics. 24 chemical components were characterized in fermented Ziziphi Spinosae Semen(FSZR). The 7 differential marker compounds between SZR and FSZR were rapidly discovered by untargeted chemomics-based mining approach using OPLS-DA. Meanwhile, the relative contents of 24 chemical components were analyzed by t-Test, which was used to make metabolic pathway analysis, showing that deacetylate and deglycosylation were the major metabolic reaction under fermentation by Poria cocos. The result shows that the proposed strategy meets the technical demands for the overall chemical characterization of fermented herbal medicines, and therefore may be considered a promising approach for delving into the scientific basis in traditional fermentation of herbal medicines.

Key words: Ziziphi Spinosae Semen, Poria cocos(Schw.) Wolf., Fermentation, UPLC-Q-Orbitrap MS/MS, Biotransformation

中图分类号: