高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (7): 1277.

• 研究论文 • 上一篇    下一篇

以氨基酸为配体的血液灌流用内毒素吸附剂的制备及性能研究

侯光辉1, 王慧彦1, 刘涛1, 袁直1, 房杰2, 李冬华2, 邱奇2, 郭世铎2   

  1. 1. 吸附分离功能高分子材料国家重点实验室,南开大学高分子化学研究所,天津300071;
    2. 天津市中西医结合急腹症研究所,天津300100
  • 收稿日期:2004-06-02 出版日期:2005-07-10 发布日期:2005-07-10
  • 通讯作者: 袁直(1961年出生),女,博士,教授,博士生导师,从事生物医用高分子材料研究.E-mail:zhiy@nankai.edu.cn E-mail:zhiy@nankai.edu.cn
  • 基金资助:

    国家“九七三”计划项目(批准号:G1999064707);国家自然科学基金(批准号:50373021);博士学科点基金(批准号:20030055038);南开大学科技创新基金资助

Preparation of Adsorbents for Endotoxin Removal in Hemoperfusion withAmino Acid as the Ligand and Investigations on Their Properties

HOU Guang-Hui1, WANG Hui-Yan1, LIU Tao1, YUAN Zhi1, FANG Jie2, LI Dong-Hua2, QIU Qi2, GUO Shi-Duo2   

  1. 1. State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China;
    2. Institute of Acute Abdomen of Integrated Traditional and Western Medicine, Tianjin 300100, China
  • Received:2004-06-02 Online:2005-07-10 Published:2005-07-10

PDF (PC)

被引次数

20

摘要： 以琼脂凝胶为载体,氨基酸为配体,合成了内毒素吸附剂,并对其吸附效果进行了比较.对吸附剂的各种性质进行了表征,选取琼脂-赖氨酸吸附剂对内毒素血症的兔子进行血液灌流,可将血浆中的内毒素降至接近正常值,最高清除率达73.6%,且吸附剂具有良好的血液相容性.

关键词: 内毒素, 琼脂凝胶, 氨基酸, 血液灌流

Abstract: Endotoxermia is caused by endotoxin, lipopolysaccharides(LPS) released from gram-negative bacteria cell wall. Endotoxin is composed of a hydrophilic polysaccharide moiety and a hydrophobic lipid moiety(lipid A) that anchors the molecules in the outer membrane. The sugar residues may be partially phosphorylated and serious endotoxin intoxication can cause sepsis and septic shocks, leading to severe hypertension, cardiovascular collapse, multiple organ failure and death. Removal of endotoxin duly and effectually from patients' blood is very important in clinic. In this paper, with agar gel as the carrier and amino acids as the ligands, adsorbents for endotoxin were prepared and their adsorption capacity for endotoxin were compared. The properties of adsorbents were investigated. The adsorbent with L-lysine as the ligand was chosen to be used for hemoperfusion. The adsorbent can remove plasma endotoxin of endotoxemia rabbit model to close to the normal level and the highest clearance percentage reached 73.6%. The adsorbent has a good blood compatibility.

Key words: Endotoxin, Agar gel, Amino acid, Hemoperfusion

中图分类号: 

TrendMD: