高等学校化学学报

高等学校化学学报

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魔角旋转核磁共振代谢组学方法对镧、铈急性生物效应的比较研究

廖沛球, 吴惠丰, 张晓宇, 李晓晶, 李中峰, 李伟生, 吴亦洁, 裴奉奎   

  1. 中国科学院长春应用化学研究所, 长春 130022
  • 收稿日期:2005-07-05 修回日期:1900-01-01 出版日期:2006-08-10 发布日期:2006-08-10
  • 通讯作者: 裴奉奎

Comparative Investigation on Acute Biological Effect of Lanthanum and Cerium by MAS 1H NMR-based Metabonomic Approach

LIAO Pei-Qiu, WU Hui-Feng, ZHANG Xiao-Yu, LI Xiao-Jing, LI Zhong-Feng, LI Wei-Sheng, WU Yi-Jie, PEI Feng-Kui   

  1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2005-07-05 Revised:1900-01-01 Online:2006-08-10 Published:2006-08-10
  • Contact: PEI Feng-Kui

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被引次数

10

摘要： 利用高分辨魔角旋转核磁共振(MAS 1H NMR)技术对腹腔注射不同剂量[2, 10, 50 mg/kg(体重)]的硝酸镧[La(NO3)3]和硝酸铈[Ce(NO3)3] 的雄性Wistar大鼠肝、肾组织的MAS 1H NMR谱进行比较分析, 研究了La(NO3)3和Ce(NO3)3的急性生物效应, 并结合模式识别技术对不同剂量La(NO3)3和Ce(NO3)3的急性生物效应进行了分类. 研究结果表明, La(NO3)3对大鼠的急性毒性主要表现为肝毒, Ce(NO3)3对大鼠肝、肾同时造成损伤. 该方法可用于其它稀土及金属化合物的毒性预测和毒理学研究.

关键词: 魔角旋转核磁共振, 代谢组学, 组织, 稀土, 生物效应

Abstract: High-resolution magic-angle-spinning(MAS) 1H NMR spectroscopic and pattern recognition(PR) based methods were applied to compare the different acute biochemical effects between La(NO3)3- and Ce(NO3)3-treated rats. Male Wistar rats were treated with various doses(2, 10, 50 mg/kg body weight) of La(NO3)3 and Ce(NO3)3, and MAS 1H NMR spectra of the intact liver and kidney tissues were analyzed by using principal components analysis to extract metabolic information. The target lesion of La(NO3)3 to liver and Ce(NO3)3 to both liver and kidney were found by MAS 1H NMR-PR methods. This work illustrated that the combination of NMR and pattern recognition technique is a powerful method to study the biochemical effects induced by xenobiotics.

Key words: Magic-angle spinning NMR, Metabonomics, Tissue, Rare earths, Biological effects

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