高等学校化学学报

高等学校化学学报

• 研究简报 • 上一篇    下一篇

化学发光磁酶免疫法检测O157:H7大肠埃希菌

朱广华1, 贺艳峰1, 郭小英2, 刘和平3   

    1. 东南大学公共卫生学院, 南京 210009;
    2. 东南大学生物科学与医学工程系分子电子学国家重点实验室, 南京 210096;
    3. 江苏省疾病预防控制中心, 南京 210009
  • 收稿日期:2005-12-22 修回日期:1900-01-01 出版日期:2006-08-10 发布日期:2006-08-10
  • 通讯作者: 朱广华

Determination of Escherichia Coli. O157:H7 in Food Based on Chemiluminescent Magnetic Enzyme-linked Immunoassay

ZHU Guang-Hua1, HE Yan-Feng1, GUO Xiao-Ying2, LIU He-Ping3   

    1. College of Public Health, Southeast University, Nanjing 210009, China;
    2. State Key Laboratory for Molecular Electronics, Department of Biological Science and Medicinal Engineering, Southeast University, Nanjing 210096, China;
    3. Jiangsu Centers for Disease Prevention and Control, Naijing 210009, China
  • Received:2005-12-22 Revised:1900-01-01 Online:2006-08-10 Published:2006-08-10
  • Contact: ZHU Guang-Hua

PDF (PC)

被引次数

30

摘要： 通过自行制备的免疫磁珠结合新型发光底物(AMPPD), 改进了化学发光磁酶免疫检测法, 对人工猪肉样品中O157:H7大肠埃希菌进行了检测, 并与人工计数法进行了比较.

关键词: 免疫分析, 化学发光, 磁性微球, 大肠埃希菌

Abstract: A high sensitive chemiluminescent magnetic enzyme-linked immunoassay method was established for Escherichia coli. O157:H7 determination. The bacterium antibody was labeled by alkaline phosphatase(ALP) that catalyzed the decomposing of substrate 3-(2-spiroadamantane)-4-methoxy-4(3-phosphoryloxy)phenyl-1,2-dioxetane(AMPPD) to give the light emission. The sensitivity of the method is 8.5×104 Cell/mL with a linear range of 1.0×105—5.0×107 Cell/mL. The intra- and inter-assay CVs are 14.8% and 20.0% in pork samples, respectively. The correlation coefficient of present and the standard plate counting method is 0.981. The experiments with the spiked samples show that this method has great potential to be applied to detecting the concentration of Escherichia coli. O157:H7 in a variety of samples.

Key words: Immunoassay, Chemiluminescence, Magnetic beads, Escherichia coli.

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