高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (4): 661.doi: 10.7503/cjcu20190645

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇    下一篇

磷脂膜的相行为对氧化石墨烯与磷脂膜相互作用的影响

张晓菲1,2,武烈1,*(),李姗姗1,2,朱曼毓1,2,程晓伟1,2,姜秀娥1,2,*()   

  1. 1. 中国科学院长春应用化学研究所, 电分析化学国家重点实验室, 长春 130022
    2. 中国科学技术大学应用化学与工程学院, 安徽 230026
  • 收稿日期:2019-12-10 出版日期:2020-04-10 发布日期:2020-02-07
  • 通讯作者: 武烈,姜秀娥 E-mail:lwu@ciac.ac.cn;jiangxiue@ciac.ac.cn
  • 基金资助:
    国家自然科学基金(21705146, 21675149)

Effect of Phase Behavior of Phospholipids on Lipid Membrane Damage Induced by Graphene Oxide

ZHANG Xiaofei1,2,WU Lie1,*(),LI Shanshan1,2,ZHU Manyu1,2,CHENG Xiaowei1,2,JIANG Xiu’e1,2,*()   

  1. 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
    2. School of Applied Chemistry and Engineering, University of Science and Technology of China, Anhui 230026, China
  • Received:2019-12-10 Online:2020-04-10 Published:2020-02-07
  • Contact: Lie WU,Xiu’e JIANG E-mail:lwu@ciac.ac.cn;jiangxiue@ciac.ac.cn
  • Supported by:
    † Suppored by the National Natural Science Foundation of China(21705146, 21675149)

摘要:

通过使用不同相变温度的磷脂分子并调节二者的比例构筑了不同相态的磷脂膜, 并利用表面增强红外光谱和激光共聚焦显微镜研究了磷脂膜的相行为对氧化石墨烯和磷脂膜相互作用的影响. 结果表明, 氧化石墨烯对磷脂膜中磷脂分子的抽提作用具有显著的相态选择性, 其选择性地抽提流动相的磷脂分子; 氧化石墨烯对流动相磷脂的抽提作用受到膜中凝胶相磷脂存在比例的影响, 只有在流动相磷脂分子占磷脂膜中磷脂分子的绝大部分时才能够发生抽提作用, 且只有流动相的磷脂分子被抽提.

关键词: 磷脂膜, 相行为, 氧化石墨烯, 磷脂抽提效应

Abstract:

The difference among different kinds of phospholipids in biomembrane not only lies in different structures in polar head groups, but also in different alkyl chain structures which lead to different phase states. Phase state of phospholipids plays an important role in cell activities. In this study, phospholipid membranes with different phase states were constructed by using two phospholipids with different phase transition temperatures and adjusting their mixing ratio, to reveal the effect of the phase behavior of the phospholipid membrane on the interaction between graphene oxide and the phospholipid membrane. Results showed that, for the extraction of phospholipid from membrane, graphene oxide exhibited significant phase selectivity, and it selectively extracted phospholipids in fluid phase. More importantly, the extraction effect of graphene oxide on fluid-phase phospholipids was affected by the presence of gel-phase phospholipids in the membrane. The extraction could occur only when the fluid-phase phospholipid molecules accounted for the majority of the phospholipids in the membrane, and only the phospholipids in fluid phase were extracted. Therefore, the extraction effect of graphene oxide on phospholipids in membrane not only had fluid-phase selectivity, but also was regulated by the phase behavior of phospholipid membrane.

Key words: Phospholipid membrane, Phase behavior, Graphene oxide, Lipid extraction effect

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