高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (5): 876-882.doi: 10.7503/cjcu20170701

• 分析化学 • 上一篇    下一篇

电化学电流校正法定量测定五羟色胺

李元1, 王婷婷1, 李梅1, 谷飞1, 鲍昌昊1, 黄蓉萍1, 马静芳1, 程寒1,2   

  1. 1. 中南民族大学药学院
    2. 民族药学国家级实验教学示范中心, 武汉 430074
  • 收稿日期:2017-11-06 出版日期:2018-04-08 发布日期:2018-04-08
  • 作者简介:

    联系人简介: 程 寒, 女, 博士, 副教授, 主要从事药物分析方面的研究. E-mail: chenghan@mail.scuec.edu.cn

  • 基金资助:
    国家自然科学基金(批准号: 21205144)、 中南民族大学学术团队资助项目(批准号: XTZ15013)、 中央高校基本科研业务费(批准号: CZZ16004)、 中南民族大学研究生学术创新基金(批准号: 2018SYCXJJ214)和中南民族大学大学生创新训练计划(批准号: 201710524029)资助.

Quantitative Determination of 5-HT by Electrochemical Current-correction Method

LI Yuan1, WANG Tingting1, LI Mei1, GU Fei1, BAO Changhao1, HUANG Rongping1, MA Jingfang1, CHENG Han1,2,*   

  1. 1. College of Pharmacy, 2. National Demonstration Center for Experimental Ethnopharmacology Education, South-Central University for Nationalities, Wuhan 430074, China
  • Received:2017-11-06 Online:2018-04-08 Published:2018-04-08
  • Contact: CHENG Han
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21205144), the Academic Team Funding Program of South-Central University for Nationalities, China(No.XTZ15013), the Fundamental Research Funds for the Central Universities of China(No.CZZ16004), the Graduate Student Innovation Fund Program of South-Central University for Nationalities, China(No.2018SYCXJJ214) and the Student Innovation and Venture Training Program of South-Central University for Nationalities, China(No.201710524029).

摘要:

制备了碳纤维超微电极, 将其在1.0×10-4 mol/L五羟色胺溶液中循环伏安扫描10圈后, 测定电极在铁氰化钾、 多巴胺等电活性溶液中的循环伏安以及差分脉冲的峰电流变化, 结果表明五羟色胺对碳纤维超微电极有毒化效应. 采用火焰灼烧法对被五羟色胺毒化的电极进行活化, 以电极在1.0×10-3 mol/L铁氰化钾溶液中的峰电流为基准, 活化后电极与原电极在铁氰化钾溶液中的峰电流之比为校正因子, 对电极的活性面积进行校正. 实验结果表明, 校正后的五羟色胺氧化峰电流与其浓度在1.0×10-6~2.0×10-4 mol/L范围内呈现良好的线性关系, 线性回归方程为I=0.2073c+0.3074, 相关系数R2=0.9962. 采用该方法对五羟色胺样品浓度进行了测定, 回收率达到98.0%~102.4%.

关键词: 碳纤维超微电极, 火焰灼烧, 活化, 电流校正法, 五羟色胺

Abstract:

In this paper, the carbon fiber ultramicroelectrode(CFUME) was prepared, after cyclic voltammetry scanning in 1.0×10-4 mol/L 5-hydroxytryptamine(5-HT), and the electrochemical behaviors of the electrode in ferricyanide and dopamine(DA) were investigated. The experimental results show that the surface of carbon fiber ultramicroelectrode has been passivated by the oxidation products of hydroxytryptamine. The carbon fiber of the deteriorated ultramicroelectrodes was burned in the faint flame to activate the surface, and the peak current ratio of the activated electrode and the new electrode in 1.0×10-3 mol/L ferricyanide solution as the correction factor, the active area of the electrode is corrected by dividing the correction factor. The experimental results show that corrected peak current and concentration of 5-HT in the range of 1.0×10-6—2.0×10-4 mol/L show a good linear relationship, and the liner regression equation is I=0.2073c+0.3074, with the related coefficient of R2=0.9962. The concentration of 5-HT was determined by this method and the recovery is 98.0%—102.4%. The method is simple and reproducible, and may be used to detect 5-HT in biological sample.

Key words: Carbon fiber ultramicroelectrode, Flame-burned, Activation, Current correction method, 5-Hydroxytryptamine

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