电化学电流校正法定量测定五羟色胺

doi:10.7503/cjcu20170701

高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (5): 876.doi: 10.7503/cjcu20170701

电化学电流校正法定量测定五羟色胺

李元¹, 王婷婷¹, 李梅¹, 谷飞¹, 鲍昌昊¹, 黄蓉萍¹, 马静芳¹, 程寒^1,²

1. 中南民族大学药学院
2. 民族药学国家级实验教学示范中心, 武汉 430074

收稿日期:2017-11-06 出版日期:2018-04-08 发布日期:2018-04-08
作者简介:
联系人简介: 程寒, 女, 博士, 副教授, 主要从事药物分析方面的研究. E-mail: chenghan@mail.scuec.edu.cn
基金资助:
国家自然科学基金(批准号: 21205144)、中南民族大学学术团队资助项目(批准号: XTZ15013)、中央高校基本科研业务费(批准号: CZZ16004)、中南民族大学研究生学术创新基金(批准号: 2018SYCXJJ214)和中南民族大学大学生创新训练计划(批准号: 201710524029)资助.

Quantitative Determination of 5-HT by Electrochemical Current-correction Method^†

LI Yuan¹, WANG Tingting¹, LI Mei¹, GU Fei¹, BAO Changhao¹, HUANG Rongping¹, MA Jingfang¹, CHENG Han^1,^2,^*

1. College of Pharmacy, 2. National Demonstration Center for Experimental Ethnopharmacology Education, South-Central University for Nationalities, Wuhan 430074, China

Received:2017-11-06 Online:2018-04-08 Published:2018-04-08
Contact: CHENG Han
Supported by:
† Supported by the National Natural Science Foundation of China(No.21205144), the Academic Team Funding Program of South-Central University for Nationalities, China(No.XTZ15013), the Fundamental Research Funds for the Central Universities of China(No.CZZ16004), the Graduate Student Innovation Fund Program of South-Central University for Nationalities, China(No.2018SYCXJJ214) and the Student Innovation and Venture Training Program of South-Central University for Nationalities, China(No.201710524029).

摘要/Abstract

摘要：

制备了碳纤维超微电极, 将其在1.0×10^-4 mol/L五羟色胺溶液中循环伏安扫描10圈后, 测定电极在铁氰化钾、多巴胺等电活性溶液中的循环伏安以及差分脉冲的峰电流变化, 结果表明五羟色胺对碳纤维超微电极有毒化效应. 采用火焰灼烧法对被五羟色胺毒化的电极进行活化, 以电极在1.0×10^-3 mol/L铁氰化钾溶液中的峰电流为基准, 活化后电极与原电极在铁氰化钾溶液中的峰电流之比为校正因子, 对电极的活性面积进行校正. 实验结果表明, 校正后的五羟色胺氧化峰电流与其浓度在1.0×10^-6~2.0×10^-4 mol/L范围内呈现良好的线性关系, 线性回归方程为I=0.2073c+0.3074, 相关系数R²=0.9962. 采用该方法对五羟色胺样品浓度进行了测定, 回收率达到98.0%~102.4%.

关键词: 碳纤维超微电极, 火焰灼烧, 活化, 电流校正法, 五羟色胺

Abstract:

In this paper, the carbon fiber ultramicroelectrode(CFUME) was prepared, after cyclic voltammetry scanning in 1.0×10^-4 mol/L 5-hydroxytryptamine(5-HT), and the electrochemical behaviors of the electrode in ferricyanide and dopamine(DA) were investigated. The experimental results show that the surface of carbon fiber ultramicroelectrode has been passivated by the oxidation products of hydroxytryptamine. The carbon fiber of the deteriorated ultramicroelectrodes was burned in the faint flame to activate the surface, and the peak current ratio of the activated electrode and the new electrode in 1.0×10^-3 mol/L ferricyanide solution as the correction factor, the active area of the electrode is corrected by dividing the correction factor. The experimental results show that corrected peak current and concentration of 5-HT in the range of 1.0×10^-6—2.0×10^-4 mol/L show a good linear relationship, and the liner regression equation is I=0.2073c+0.3074, with the related coefficient of R²=0.9962. The concentration of 5-HT was determined by this method and the recovery is 98.0%—102.4%. The method is simple and reproducible, and may be used to detect 5-HT in biological sample.

Key words: Carbon fiber ultramicroelectrode, Flame-burned, Activation, Current correction method, 5-Hydroxytryptamine

中图分类号:

O657.1

TrendMD:

李元, 王婷婷, 李梅, 谷飞, 鲍昌昊, 黄蓉萍, 马静芳, 程寒. 电化学电流校正法定量测定五羟色胺. 高等学校化学学报, 2018, 39(5): 876.

LI Yuan,WANG Tingting,LI Mei,GU Fei,BAO Changhao,HUANG Rongping,MA Jingfang,CHENG Han. Quantitative Determination of 5-HT by Electrochemical Current-correction Method^†. Chem. J. Chinese Universities, 2018, 39(5): 876.

图/表 9

Fig.1 Voltammogram curves of clean carbon fiber ultramicroelectrode in 1×10-4 mol/L 5-HT solution Note:(A) 10 scans of cyclic voltammetry; (B) 10 scans of differential pulse voltammetry.

Table 1 Peak current attenuation levels of CFUME in 1.0×104 mol/L 5-HT solution during poisoning*

Number of scan cycles	1	2	3	4	5	6	7	8	9	10
Peak current of CV/nA	36.37	16.78	11.62	6.58	4.59	4.01	2.50	1.73	1.53	0.97
Peak current decay level of CV(%)	100.00	46.14	31.95	18.09	12.62	11.03	6.87	4.76	4.21	2.67
Peak current of DPV/nA	13.36	9.27	7.26	6.13	5.10	4.39	3.82	3.35	2.97	2.60
Peak current decay level of DPV(%)	100.00	69.39	54.34	45.88	38.17	32.86	28.59	25.08	22.23	19.46

Fig.2 Electrochemical behavior of CFUME in 1.0×10-3 mol/L potassium ferricyanide solution(A, B) and in 1.0×10-4 mol/L dopamine solution(C, D) Note:(A) and (C) Cyclic voltammograms; (B) and (D) differential pulse voltammograms. a. Before poisoning; b. after poisoning; c. after activation.

Fig.3 SEM images of a clean CFUME(A), a CFUME after poisoning(B) and a CFUME after activation(C)

Fig.4 DPV overlay chart of CFUME in 1.0×10-3 mol/L potassium ferricyanide solution

Fig.5 Differential pulse voltammograms of CFUME in 1.0×10-3 mol/L potassium ferricyanide solution(A) and in gradient concentrations of 5-HT solution(B) Note: (A) a—h: Scanning sequence in K3[Fe(CN)6] solution; (B) a—g: 1.0×10-6, 5.0×10-6, 1.0×10-5, 5.0×10-5, 1.0×10-4, 1.5×10-4 and 2.0×10-4 mol/L, respectively.

Table 2 Correction of oxidation peak current for carbon fiber ultramicroelectrode in various concentrations of 5-HT(n=5)

Electrode state	c^a/(μmol·L^-1)	I^b/nA	I^c/nA	Correction factor	I^d/nA	RSD(%)
Clean electrode	1.0	45.04	0.30	1.00	0.30	2.4
The first activation	5.0	39.52	0.96	0.88	1.10	3.1
The second activation	10.0	29.08	1.86	0.65	2.86	3.5
The third activation	50.0	29.04	7.67	0.64	11.98	4.2
The fourth activation	100.0	22.75	9.82	0.51	19.25	2.7
The fifth activation	150.0	19.78	13.66	0.44	31.05	1.9
The sixth activation	200.0	18.53	17.46	0.41	42.59	1.8

Fig.6 Relationship of oxidation peak current and concentration of 5-HT before(a) and after current correction(b)

Table 3 Determination result of 5-HT in mouse serum samples(n=5)

Sample	10⁵Initial value/(mol·L^-1)	10⁵Spiked/(mol·L^-1)	10⁵Found/(mol·L^-1)	Recovery(%)
1	1.00	0.50	1.54	102.7
2	1.00	1.00	1.91	95.5
3	1.00	1.50	2.61	104.4
4	1.00	2.00	2.93	97.7
5	1.00	3.00	4.12	103.0

参考文献 26

[1]	Morgan L.D., Baker H., Yeoman M. S., Patel B. A., Analyst, 2012, 137(6), 1409—1415
[2]	Wacker D., Wang S., Mccorvy J.D., Betz R. M., Venkatakrishnan A. J., Levit A., Cell, 2017, 168(3), 377—389
[3]	Erland L., Saxena P.K., Neurotransmitter, 2017, 4, 1538—1550
[4]	Liao X.J., Mao W. M., Wu W. J., Deng Q., Yang G. G., Chinese Archies of Traditional Chinese Medicine, 2013, 31(2), 355—357
	(廖秀军, 茅伟明, 武文静, 邓群, 杨关根. 中华中医药学刊, 2013, 31(2), 355—357)
[5]	Anderluh A., Hofmaier T., Klotzsch E., Kudlacek O., Stockner T., Sitte H. H., Nat. Commun., 2017, 8, 14089—14096
[6]	David E., Nichols., Charles D., Nichols., Chem. Rev., 2008, 108(5), 1614—1641
[7]	Muller C., Jacobs B., Handbook of the Behavioural Neurobiology of Serotonin, Elsevier Science and Technology, New York, 2009
[8]	Khan N.A., Troutaud D., Moulinoux J. H., Deschaux P., Neurosci. Res. Commun., 2015, 18(2), 97—105
[9]	Cai W., Khaoustov V.I., Xie Q., Pan T., Le W., Yoffe B., [J]. Hepatol., 2015, 42(6), 880—887
[10]	Fang H., Vickrey T.L., Venton B. [J]., Anal. Chem., 2011, 83(6), 2258—2264
[11]	Güell A.G., Meadows K. E., Unwin P. R., Macpherson J. V., Phys. Chem. Chem. Phys., 2010, 12(34), 10108—10114
[12]	Hu C., Liu F., Lan H., Liu H., Qu J., [J]. Colloid Interf. Sci., 2015, 446, 359—365
[13]	Zhang B., Heien M.L., Santillo M. F., Mellander L., Ewing A. G., Anal. Chem., 2011, 83, 571—577
[14]	Cheng H., Huang W.H., Chen R. H., Wang Z. L., Cheng J. K., Electrophoresis, 2007, 28, 1579—1586
[15]	Liu X., Syed B., Wonchul S., Cherian J., Mathai., Shubhra G., Kevin D., Gillis., Anal. Chem., 2011, 83, 2445—2451
[16]	Baganz N.L., Horton R. E., Martin K. P., Holmes A., Daws L. C., [J]. Neurosci., 2010 30(45), 15185—15195
[17]	Stephens M.L., Francois P., Peter H., Gerhardt G. A., Zhiming Z., [J]. Neurosci. Meth., 2010, 185(2), 264—283
[18]	Njagi J., Ball M., Best M., Wallace K.N., Andreescu S., Anal. Chem., 2010, 82(5), 1822—1830
[19]	Takmakov P., Zachek M.K., Keithley R. B., Walsh P. L., Donley C., Mccarty G. S., Anal. Chem., 2010, 82(5), 2020—2028
[20]	Strand A.M., Venton B. [J]., Anal. Chem., 2008, 80(10), 3708—3715
[21]	Jackson B.P., Dietz S. M., Wightman R. M., Anal. Chem., 1995, 67(6), 1115—1120
[22]	Cheng H., Lan J.F., Wei G. H., Huang W. H., Cheng J. K., Chinese [J]. Anal. Chem., 2013, 41(4), 540—545
	(程寒, 兰嘉峰, 韦光汉, 黄卫华, 程介克. 分析化学, 2013, 41(4), 540—545)
[23]	Hashemi P., Dankoski E.C., Petrovic J., Keithley R. B., Wightman R. M., Anal. Chem., 2009, 81(22), 9462—9471
[24]	Zhu M.F., The New Electrochemical Analysis Method Study for the Determination of Neurotransmitters, South China University of Technology, Guangzhou, 2012(朱明芳. 神经递质分子电化学测定的新方法研究, 广州: 华南理工大学, 2012)
[25]	Xun Y., Cao Z., Song T.M., Lü C. Z., Liu F., He J. L., Yang R. H., Chem. [J]. Chinese Universities, 2016, 37(5), 835—843
	(寻艳, 曹忠, 宋天铭, 吕超志, 刘峰, 何婧琳, 杨荣华. 高等学校化学学报, 2016, 37(5), 835—843)
[26]	Wang J.N., Su P., Li D., Wang T., Yang Y., Chem. Res. Chinese Universities, 2017, 33(4), 540—545

电化学电流校正法定量测定五羟色胺

Quantitative Determination of 5-HT by Electrochemical Current-correction Method^†

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 26

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王红宁, 黄丽, 清江, 马腾洲, 蒋伟, 黄维秋, 陈若愚. 香蒲基生物炭的活化及对VOCs吸附的应用[J]. 高等学校化学学报, 2022, 43(4): 20210824.
[2]	何宇婧, 李佳乐, 王东洋, 王福玲, 肖作旭, 陈艳丽. 锌活化Fe/Co/N掺杂的生物质碳基高效氧还原催化剂[J]. 高等学校化学学报, 2022, 43(11): 20220475.
[3]	周敏, 石莹莹, 李树奇, 张凯林, 崔永亮, 张森, 张先燚, 孔祥蕾. 不同电荷态泛素蛋白离子的193 nm紫外光解离质谱[J]. 高等学校化学学报, 2021, 42(8): 2436.
[4]	赵东霞, 张海霞, 冯文娟, 杨忠志. 分子形貌所指示的羟基卡宾及其衍生物的质子转移反应[J]. 高等学校化学学报, 2021, 42(7): 2187.
[5]	王乙舒, 李雪, 闫丽, 徐红赟, 祝玉鑫, 宋艳华, 崔言娟. 二维Z型BCN/Sn₃O₄复合材料的光催化还原性能[J]. 高等学校化学学报, 2021, 42(12): 3722.
[6]	周春妮, 郑子昂, 彭望明, 王洪波, 张玉敏, 王亮, 肖标. 微波辅助下铑催化二芳基膦酰胺与炔烃的C—H活化/环化反应[J]. 高等学校化学学报, 2020, 41(4): 726.
[7]	李博文,汪若蘅,黎丽,肖杨. 碱活化多孔碳用于分离甲苯及活化/吸附机理[J]. 高等学校化学学报, 2020, 41(2): 284.
[8]	张林, 张尉, 岳鑫, 李鹏杰, 杨作银, 蒲敏, 雷鸣. 锰配合物催化CO₂加氢生成甲酸的理论研究[J]. 高等学校化学学报, 2019, 40(9): 1911.
[9]	蔡利海, 郭宝华, 张诚, 徐军, 黄忠耀. 以时温等效方法研究尼龙1010应力松弛行为及使用寿命预测[J]. 高等学校化学学报, 2019, 40(4): 832.
[10]	马俊锋, 曾毅. Au纳米颗粒负载SnO₂双层空心立方体的CO气敏性能[J]. 高等学校化学学报, 2018, 39(9): 1867.
[11]	杨梦, 张腾烁, 郑旭明, 薛佳丹. 2-硝基萘激发态衰减动力学和光解通道的从头计算研究[J]. 高等学校化学学报, 2018, 39(8): 1734.
[12]	方升, 王梅艳, 刘静静, 刘靖尧. 镍配合物催化N-烯丙基酰胺异构化反应机理[J]. 高等学校化学学报, 2018, 39(7): 1475.
[13]	高俊, 胡辉, 刘雪岩. 改性蓝藻基活性炭的制备及常压下对CO₂的捕集性能[J]. 高等学校化学学报, 2018, 39(2): 284.
[14]	王昀, 贲腾. 海藻基含杂原子碳材料的制备及电化学性质[J]. 高等学校化学学报, 2018, 39(12): 2627.
[15]	王昀, 贲腾. 海藻基微孔碳材料的储甲烷性能[J]. 高等学校化学学报, 2018, 39(10): 2143.

电化学电流校正法定量测定五羟色胺

Quantitative Determination of 5-HT by Electrochemical Current-correction Method†

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 26

相关文章 15

编辑推荐

Metrics

本文评价

Quantitative Determination of 5-HT by Electrochemical Current-correction Method^†