高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (5): 938.doi: 10.7503/cjcu20150943

• 物理化学 • 上一篇    下一篇

基于硼酸-二醇特异性识别作用的层层组装薄膜对肌红蛋白的吸入及其电化学研究

姚惠琴1(), 黄珊1, 苏巧玲1, 史可人2, 甘倩倩1, 王明科1   

  1. 1. 宁夏医科大学药学院, 基础医学院, 银川 750004
    2. 宁夏大学能源化工国家重点实验室, 银川 750021
  • 收稿日期:2015-12-10 出版日期:2016-05-10 发布日期:2016-04-22
  • 作者简介:联系人简介: 姚惠琴, 女, 博士, 副教授, 主要从事生物/药物电分析化学研究. E-mail:huiqin_yao@163.com
  • 基金资助:
    高等学校科学研究项目(批准号: NGY2014101)和国家级大学生创新实验计划项目(批准号: 20140315)资助

Loading of Myoglobin into Layer-by-layer Films Assembled Through Boronic Acid-diol Specific Recognition and Its Electrochemical Study

YAO Huiqin1,*(), HUANG Shan1, SU Qiaoling1, SHI Keren2,*, GAN Qianqian1, WANG Mingke1   

  1. 1. College of Pharmacy, College of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China
    2. State Key Laboratory of Energy Sources & Engineering, Ningxia University, Yinchuan 750021, China
  • Received:2015-12-10 Online:2016-05-10 Published:2016-04-22
  • Contact: YAO Huiqin,SHI Keren E-mail:huiqin_yao@163.com

摘要:

合成了既含有苯硼酸(PBA)基团又含有羧酸基团的聚电解质PAA-PBA(PAA: 聚丙烯酸). 采用层层组装(LbL)技术, 利用PAA-PBA和葡聚糖(Dex)之间的硼酸-二醇特异性识别作用, 在热解石墨电极(PG)表面构筑了{PAA-PBA/Dex}nLbL薄膜, 该薄膜从溶液中吸入肌红蛋白(Mb)形成{PAA-PBA/Dex}n-Mb薄膜. 采用循环伏安方法研究了{PAA-PBA/Dex}n薄膜中Mb的直接电化学及对氧气和过氧化氢的电催化还原过程. 结果表明, 该薄膜为保持Mb的生物活性提供了良好的微环境, 是一种新型的可固定蛋白质的LbL薄膜, 为设计基于酶的直接电化学生物传感器提供了新思路.

关键词: 直接电化学, 电催化, 层层组装, 硼酸-二醇特异性识别作用, 肌红蛋白

Abstract:

A polyelectrolyte PAA-PBA containing both phenylboronic acid(PBA) moieties and carboxylic acid groups[PAA=poly(acrylic acid)] was synthesized. PAA-PBA and dextran(Dex) were then assembled into {PAA-PBA/Dex}n layer-by-layer(LbL) films by LbL assemble technique on pyrolytic graphite(PG) electrode surface through boronic acid-diol specific recognition between them. Myoglobin(Mb) in a buffer solution(pH=5.0) was loaded into the films, forming stable {PAA-PBA/Dex}n-Mb films. By cyclic voltammetry(CV) technique, the {PAA-PBA/Dex}n-Mb film electrodes demonstrated the direct electrochemistry of Mb and could be used to electrocatalyze reduction of various substrates(e.g. O2 and H2O2).

Key words: Direct electrochemistry, Electrocatalysis, Layer-by-layer assembly, Boronic acid-diol specific recognition, Myoglobin

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