拟南芥转酮醇酶蛋白AtTKL1的同源建模及与α-三联噻吩结合作用分析

doi:10.7503/cjcu20140934

高等学校化学学报 ›› 2015, Vol. 36 ›› Issue (4): 682.doi: 10.7503/cjcu20140934

拟南芥转酮醇酶蛋白AtTKL1的同源建模及与α-三联噻吩结合作用分析

赵斌^1,², 霍静倩¹, 邢继红², 齐萌¹, 张金林^1,²(), 董金皋²()

1. 河北农业大学植物保护学院
2. 真菌毒素与植物分子病理学实验室, 保定 071000

收稿日期:2014-10-22 出版日期:2015-04-10 发布日期:2015-03-19
作者简介:联系人简介: 张金林, 男, 博士, 教授, 博士生导师, 主要从事天然产物农药研究. E-mail: zhangjinlin@hebau.edu.cn. 董金皋, 男, 博士, 教授, 博士生导师, 主要从事植物病原真菌毒素研究. Email: dongjingao@126.com
基金资助:
国家自然科学基金(批准号: 31171877)和河北省自然科学基金(批准号: C2012204006)资助

Homologous Modeling of Transketolase AtTKL1 and Its Combination with α-Terthienyl in Arabidopsis Thaliana^†

ZHAO Bin^1,², HUO Jingqian¹, XING Jihong², QI Meng¹, ZHANG Jinlin^1,^2,^*(), DONG Jingao^2,^*()

1. College of Plant Protection
2. Mycotoxin and Molecular Plant Pathology Laboratory,Agricultural University of Hebei, Baoding 071001, China

Received:2014-10-22 Online:2015-04-10 Published:2015-03-19
Contact: ZHANG Jinlin,DONG Jingao E-mail:zhangjinlin@hebau.edu.cn;dongjingao@126.com
Supported by:
† Supported by the National Natural Science Foundation of China(No.31171877) and the Natural Science Foundation of Hebei Province, China(No.C2012204006)

摘要/Abstract

摘要：

以玉米转酮醇酶(1ITZ)的晶体结构为模板, 对拟南芥转酮醇酶(AtTKL1)的三维结构进行模拟和优化. 利用生物信息学方法, 确定其氨基酸结合位点为His143, Gly234, Asn263, Arg434, Ser461, Gln488, Phe515, His539, Asp547和Arg598, 催化氨基酸位点为His103和His340. 通过分子模拟确定α-三联噻吩与AtTKL1的活性口袋可有效结合, 且AtTKL1的催化位点和结合位点为其与α-三联噻吩结合的关键位点. 利用荧光猝灭光谱技术确定AtTKL1与α-三联噻吩之间存在结合作用; 酶活力检测结果表明加入α-三联噻吩后转酮醇酶蛋白的活性降低, 进一步验证了AtTKL1与α-三联噻吩之间的相互作用.

关键词: 转酮醇酶, α-三联噻吩, 同源建模, 分子对接

Abstract:

Transketolase is the key enzyme of photosynthesis pathways. It is of great importance for herbicide seeking through new herbicidal substance targeting transketolase. In this study, homologous protein of transketolase was obtained through Modeler and optimized using Amber12, its binding sites of pivotal amino acids were identified with bioinformatics tools. Interaction between AtTKL1 and α-terthienyl finally got verified by spectroscopy and enzyme activity analysis. The three dimensional frame model of transketolase protein AtTKL1 in Arabidopsis thaliana was homologically modeled according to Maize transketolase protein 1ITZ, revised by dynamic molecular methods. With bioinformatics tools, the sites in AtTKL1 were determined, His143, Gly234, Asn263, Arg434, Ser461, Gln488, Phe515, His539, Asp547and Arg598 for binding, His103 and His340 for catalysis. The molecular docking of AtTKL1 and α-terthienyl was performed by computer simulation, and the results confirmed the perfect match of α-terthienyl with active cleavage of AtTKL1. The binding sites His143 and Gly234, and the catalytic sites His103 and His340 were the responsible binding sites of α-terthienyl. Prokaryotic expression of the protein and fluorescence quenching spectroscopy have verified the combination of α-terthienyl and AtTKL1. The reduction on the activity of transketolase treatmented with α-terthienyl gave a further confirmation about the interaction between AtTKL1 and α-terthienyl. The results will contribute to exploiting new efficient and low toxic herbicides.

Key words: Transketolase, α-Terthienyl, Homologous modeling, Molecular docking

中图分类号:

O629.7

TrendMD:

赵斌, 霍静倩, 邢继红, 齐萌, 张金林, 董金皋. 拟南芥转酮醇酶蛋白AtTKL1的同源建模及与α-三联噻吩结合作用分析. 高等学校化学学报, 2015, 36(4): 682.

ZHAO Bin, HUO Jingqian, XING Jihong, QI Meng, ZHANG Jinlin, DONG Jingao. Homologous Modeling of Transketolase AtTKL1 and Its Combination with α-Terthienyl in Arabidopsis Thaliana^†. Chem. J. Chinese Universities, 2015, 36(4): 682.

图/表 2

参考文献 23

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Final concentration/(mg·L^-1)	Max value of fluorescence/a.u.	Enzyme activity/U
20	31.43±0.31	26.07±2.14
40	29.44±0.27	22.50±2.21
60	26.97±0.14	18.31±2.00
80	24.35±0.17	13.93±1.21
100	21.61±0.28	9.16±0.77
120	19.46±0.24	6.43±1.03

Final concentration/(mg·L^-1)	Max value of fluorescence/a.u.	Enzyme activity/U
20	31.43±0.31	26.07±2.14
40	29.44±0.27	22.50±2.21
60	26.97±0.14	18.31±2.00
80	24.35±0.17	13.93±1.21
100	21.61±0.28	9.16±0.77
120	19.46±0.24	6.43±1.03

拟南芥转酮醇酶蛋白AtTKL1的同源建模及与α-三联噻吩结合作用分析

Homologous Modeling of Transketolase AtTKL1 and Its Combination with α-Terthienyl in Arabidopsis Thaliana^†

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拟南芥转酮醇酶蛋白AtTKL1的同源建模及与α-三联噻吩结合作用分析

Homologous Modeling of Transketolase AtTKL1 and Its Combination with α-Terthienyl in Arabidopsis Thaliana†

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Homologous Modeling of Transketolase AtTKL1 and Its Combination with α-Terthienyl in Arabidopsis Thaliana^†