高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (7): 1739.doi: 10.7503/cjcu20120965

• 物理化学 • 上一篇    下一篇

氯过氧化物酶修饰电极用于岩白菜素的电催化氯化

王珂, 吴霞琴, 李琳, 田海涛, 陆中庆   

  1. 上海师范大学生命与环境科学学院, 上海 200234
  • 收稿日期:2012-10-25 出版日期:2013-07-10 发布日期:2013-06-21
  • 通讯作者: 吴霞琴, 女, 教授, 主要从事生物电化学和电化学催化等方面的研究. E-mail: xqwu@shnu.edu.cn E-mail:xqwu@shnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20973114)资助.

Electrochemical Catalytic Chlorination of Bergenin by Chloroperoxidase Modified Electrode

WANG Ke, WU Xia-Qin, LI Lin, TIAN Hai-Tao, LU Zhong-Qing   

  1. College of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China
  • Received:2012-10-25 Online:2013-07-10 Published:2013-06-21

PDF (PC)

被引次数

1

摘要：

将氯过氧化物酶(CPO)与双十二烷基二甲基溴化铵(DDAB)混合后滴涂于玻碳(GC)电极表面, 制得CPO-DDAB/GC修饰电极. 循环伏安结果显示, 固定在电极表面的CPO可与电极之间发生直接的电子传递作用. 利用高效液相色谱-质谱联用技术对反应产物进行表征, 结果显示, 以该CPO修饰电极为工作电极, 在氧气饱和的氯化钾-岩白菜素溶液中, 利用CPO催化氧还原生成的过氧化氢可进一步驱动CPO对岩白菜素的催化氯化反应. 经估算总转化数(TTN)达到13600, 即1 mol CPO可催化13600 mol岩白菜素.

关键词: 氯过氧化物酶, 修饰电极, 岩白菜素, 生物酶催化, 电催化, 氯化

Abstract:

A chloroperoxidase(CPO) modified electrode was fabricated by drop-coating the mixture solution of CPO and didodecyldimethylammonium bromide(DDAB) onto the glassy carbon electrode surface. Direct electron transfer of immobilized CPO was observed from cyclic voltammogram. The CPO modified electrode was further used as the working electrode for electrocatalytic chlorination of bergenin in O2-saturated bergenin solution containing potassium chloride. The chlorinated product was confirmed by HPLC-MS. The total turnover number(TTN) of the reaction is estimated to be 13600.

Key words: Chloroperoxidase, Modified electrode, Bergenin, Biocatalysis, Electrochemical catalysis, Chlorination

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