高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

当归多糖ASP3的甲基化分析

孙元琳1,3, 申瑞玲2, 汤坚3, 顾小红3   

    1. 运城学院生命科学系, 运城 044000;
    2. 郑州轻工业学院食品与生物工程学院, 郑州 450002;
    3. 江南大学食品科学与技术国家重点实验室, 无锡 214122
  • 收稿日期:2007-11-12 修回日期:1900-01-01 出版日期:2008-07-10 发布日期:2008-07-10
  • 通讯作者: 孙元琳

Methylation Analysis of Polysaccharide ASP3 from Angelica sinensis(Oliv.) Diels

SUN Yuan-Lin1,3*, SHEN Rui-Ling2, TANG Jian3, GU Xiao-Hong3   

    1. Department of Life Science, Yuncheng University, Yuncheng 044000, China;
    2. College of Food and Biological Engineering, Zhenzhou Institute of Light Industry, Zhengzhou 450002, China;
    3. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Received:2007-11-12 Revised:1900-01-01 Online:2008-07-10 Published:2008-07-10
  • Contact: SUN Yuan-Lin

PDF (PC)

被引次数

47

摘要： 通过部分酸水解和甲基化分析, 结合GC-MS测试手段, 对当归多糖ASP3的糖链结构进行了研究. 采用0.2 mol/L三氟乙酸(Trifluoroacetic acid, TFA)对ASP3进行了部分酸水解, 对水解前后的多糖组分进行甲基化分析. 结果显示: ASP3的糖残基主要由D-GalpA, D-Galp, L-Araf和L-Rhap组成, 主链由1,4-D-GalpA连接, 形成“光滑区”半乳糖醛酸聚糖, 由1,4-D-GalpA通过O-4位与1,2-; 1,2,4-L-Rhap的O-2位交替连接形成含有较多分支的“毛发区”鼠李半乳糖醛酸聚糖. 58.8%的Rhap残基发生O-4位取代(1,2,4-L-Rhap). 由T-, 1,5-, 1,3,5-Araf和T-, 1,3-, 1,3,6-, 1,4-, 1,4,6-D-Galp聚合形成的阿拉伯半乳聚糖、半乳聚糖以及阿拉伯聚糖是ASP3侧链的主要组成, 通过Rhap残基的O-4位与主链相连.

关键词: 当归, 多糖, 甲基化分析, 部分酸水解

Abstract: The structural features of polysaccharide ASP3 were studied by partial acid hydrolysis and methylation methods, combined with GC-MS instrument. ASP3 was hydrolyzed with 0.2 mol/L trifluoroacetic acid and methylation analysis method were conducted on the samples. The results indicate that the residues of sugar constituents were mainly D-GalpA, D-Galp, L-Araf and L-Rhap. ASP3 contained the main part of homogalacturonan fragments as “smooth regions” with 1,4-D-GalpA and rhamnogalacturonan segments as “hairy regions”. A total of 58.8% Rhap residues in the backbone were substituted at O-4 position by side chains. T-, 1,5-, 1,3,5-Araf and T-, 1,6-, 1,3,6-, 1,4-, 1,4,6-D-Galp were linked as arabinogalactan, galactan and arabinan, which were the mainly constitution of the branch.

Key words: Angelica sinensis, Polysaccharides, Methylation analysis, Partial acid hydrolysis

中图分类号: 

TrendMD: