载银离子PLGA-PEG-PLGA温度敏感水凝胶的制备及体外抗菌性能

doi:10.7503/cjcu20190351

摘要/Abstract

摘要：

利用聚乙二醇(PEG 1000)引发乙交酯和 D,L-丙交酯开环共聚合, 制备了聚丙交酯乙交酯(PLGA)三嵌段共聚物(PLGA-PEG-PLGA)温敏水凝胶材料; 利用核磁共振氢谱( ¹H NMR)确定了产物的结构及组成. 通过还原硝酸银的方法制备银纳米粒子(AgNPs), 并将其与PLGA-PEG-PLGA三嵌段共聚物水凝胶混合, 制得新型AgNPs/PLGA-PEG-PLGA复合水凝胶; 对该复合水凝胶的相关性能进行了表征. AgNPs/PLGA-PEG-PLGA复合水凝胶仍然具有温敏性能, 随着温度升高可发生溶胶-凝胶的相转变; 还可以持续释放银纳米粒子, 从而发挥抗菌性能. 体外细胞实验结果表明, AgNPs/PLGA-PEG-PLGA复合水凝胶具有良好的生物相容性, 未见明显细胞毒性, 是具有应用前景的新型复合水凝胶.

关键词: PLGA-PEG-PLGA, 温度敏感水凝胶, 银纳米粒子, 抗菌性能

Abstract:

The PLGA-PEG-PLGA triblock thermosensitive hydrogel was synthesized via ring opening copolymerization of D,L-lactide(D,L-LA) and glycolide(GA) with PEG1000 as a macromolecular initiator. ¹H NMR spectrum of the copolymer was obtained to confirm the structure and composition. Silver nanoparticles which prepared by reduction of silver nitrate were mixed with the prepared PLGA-PEG-PLGA three block copolymer hydrogel. A new type of AgNPs/PLGA-PEG-PLGA hydrogel was prepared. The properties of AgNPs/PLGA-PEG-PLGA composite hydrogels were characterized. The AgNPs/PLGA-PEG-PLGA composite hydrogel still has a temperature sensitive property, and the sol-gel phase transition occurs with the increase of temperature. Novel hydrogel can be sustained release of silver nanoparticles to play antibacterial properties. In vitro biocompatibility experiments showed that the novel AgNPs/PLGA-PEG-PLGA hydrogel has good biocompatibility and no cytotoxicity. It is a new type of composite hydrogel with good application prospect.

Key words: PLGA-PEG-PLGA, Thermosensitive hydrogel, AgNPs, Antibacterial

中图分类号:

O632

TrendMD:

袁宝明,董晓明,杨帆,彭传刚,王金成,吴丹凯. 载银离子PLGA-PEG-PLGA温度敏感水凝胶的制备及体外抗菌性能. 高等学校化学学报, 2019, 40(10): 2225.

YUAN Baoming,DONG Xiaoming,YANG Fan,PENG Chuangang,WANG Jincheng,Wu Dankai. Preparation and the Antibacterial Activity of AgNPs/PLGA-PEG-PLGA Composite Hydrogel ^†. Chem. J. Chinese Universities, 2019, 40(10): 2225.

图/表 10

Fig.1 1H NMR spectrum of PLGA-PEG-PLGA in CDCl3

Fig.2 Temperature-dependent sol-gel phase transitions of the copolymer solutions Insets: (Ⅰ) sol, <35 ℃; (Ⅱ) gel, 35—65 ℃; (Ⅲ) precipition, >65 ℃.

Fig.3 In vitro mass loss profiles for the PLGA-PEG-PLGA hydrogels with polymer concentration of 25%

Fig.4 UV-Vis spectrum of AgNPs

Fig.5 XRD patterns of hydrogel(a), AgNPs(b) and AgNPs/hydrogel(c)

Fig.6 TEM images of AgNPs(A), PLGA-PEG-PLGA(B) and Ag-NPs/PLGA-PEG-PLGA(C) The arrow shows AgNPs encapsulated in PLGA-PEG-PLGA micelles.

Fig.7 Sol(A) and gel(B) phases of AgNPs/PLGA-PEG-PLGA

Fig.8 Storage moduli of the hydrogels and AgNPs/PLGA-PEG-PLGA a. 25%Gel; b. 0.0125%Ag/25%gel; c. 0.025%Ag/25%gel.

Fig.9 In vitro cytotoxicities of PLGA-PEG-PLGA gel and Ag/PLGA-PEG-PLGA composite hydrogel extracts with different AgNPs concentrations to L929 cells with PEI 25K as a positive control Data are presented as mean±standard deviation(n=3). a. 25%Gel; b. 0.00625%Ag; c. 0.0125%Ag; d. 0.025%Ag; e. 0.05%Ag; f. 0.1%Ag; g. Tris.

Fig.10 Antibacterial ring experiment of AgNPs/PLGA-PEG-PLGA composite hydrogel

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